BMRB Entry 50709
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50709
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Title: RGG Mini PubMed: 33934561
Deposition date: 2021-01-15 Original release date: 2021-11-14
Authors: Mompean, Miguel; Laurents, Douglas
Citation: Mompean, Miguel; Oroz, Javier; Laurents, Douglas. "Do polyproline II helix associations modulate biomolecular condensates?" FEBS Open Bio 11, 2390-2399 (2021).
Assembly members:
entity_1, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: XRGGYGGRGGWGGRGGYX
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 82 |
15N chemical shifts | 1 |
1H chemical shifts | 145 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RGG mini | 1 |
Entities:
Entity 1, RGG mini 18 residues - Formula weight is not available
The peptide is N-acetylated and C-amidated.
1 | ACE | ARG | GLY | GLY | TYR | GLY | GLY | ARG | GLY | GLY | ||||
2 | TRP | GLY | GLY | ARG | GLY | GLY | TYR | NH2 |
Samples:
sample_1: RGG mini 6.1 ± 0.2 mM; DSS 0.05 ± 0.005 mM
sample_conditions_1: ionic strength: 10 mM; pH: 2.7; pressure: 0.92 atm; temperature: 278.2 K
sample_conditions_2: ionic strength: 10 mM; pH: 9.95; pressure: 0.92 atm; temperature: 278.2 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1D 1H | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
1D 1H | sample_1 | isotropic | sample_conditions_2 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_2 |
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_2 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_2 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
Software:
TOPSPIN v4.0.8 - collection, processing
NMRFAM-SPARKY v3.13 - chemical shift assignment, peak picking
CYANA - structure solution
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
- Bruker AVANCE NEO 800 MHz