BMRB Entry 50736
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50736
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Title: Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease PubMed: 33929321
Deposition date: 2021-01-27 Original release date: 2021-05-18
Authors: Tzeng, Shiou-Ru
Citation: Tzeng, Shiou-Ru; Tseng, Yin-Chu; Lin, Chien-Chu; Hsu, Chia-Ying; Huang, Shing-Jong; Kuo, Yi-Ting; Chang, Chung-I. "Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease" elife 10, e64056-e64056 (2021).
Assembly members:
entity_1, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-Ig2D5-S20
Entity Sequences (FASTA):
entity_1: ASSHATRQLSGLKIHSNLYH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 31 |
| 15N chemical shifts | 17 |
| 1H chemical shifts | 17 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sul20 in-NTD bound state | 1 |
Entities:
Entity 1, Sul20 in-NTD bound state 20 residues - Formula weight is not available
| 1 | ALA | SER | SER | HIS | ALA | THR | ARG | GLN | LEU | SER | |
| 2 | GLY | LEU | LYS | ILE | HIS | SER | ASN | LEU | TYR | HIS |
Samples:
sample_1: Sul20 in-NTD bound state, [U-98% 15N], 0.05 mM; Sul20 in-NTD bound state, [U-99% 13C; U-99% 15N], 0.3 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker Bruker AVIII-800 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts