BMRB Entry 50739

Name: Backbone 1H, 13C, and 15N Chemical Shift assignments for the +7K+12D mutant of hnRNPA1-LCD
Published: 2022-04-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50739

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Chemical Shift assignments for the +7K+12D mutant of hnRNPA1-LCD PubMed: 34931046

Deposition date: 2021-01-29 Original release date: 2022-04-15

Authors: Borcherds, Wade

Citation: Bremer, Anne; Farag, Mina; Borcherds, Wade; Peran, Ivan; Martin, Erik; Pappu, Rohit; Mittag, Tanja. "Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains" Nat. Chem. 14, 196-207 (2022).

Assembly members:
entity_1, polymer, 137 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDest17

Entity Sequences (FASTA):
entity_1: GSMASADSSQRDRDDKGNFG DGRGGGFGGNDNFGRGGNFS DRGGFGGSRGDGKYGGDGDK YNGFGNDGKNFGGGGSYNDF GNYNNQSSNFDPMKGGNFKD RSSGPYDKGGQYFAKPRNQG GYGGSSSSKSYGSDRRF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	334
15N chemical shifts	128
1H chemical shifts	128

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Low complexity domain (LCD)	1

Entities:

Entity 1, Low complexity domain (LCD) 137 residues - Formula weight is not available

G1 to F137

1

GLY

SER

MET

ALA

SER

ALA

ASP

SER

GLN

2

ARG

ASP

ARG

ASP

LYS

GLY

ASN

PHE

GLY

3

ASP

GLY

ARG

GLY

PHE

GLY

ASN

4

ASP

ASN

PHE

GLY

ARG

GLY

ASN

PHE

SER

5

ASP

ARG

GLY

PHE

GLY

SER

ARG

GLY

6

ASP

GLY

LYS

TYR

GLY

ASP

GLY

ASP

LYS

7

TYR

ASN

GLY

PHE

GLY

ASN

ASP

GLY

LYS

ASN

8

PHE

GLY

SER

TYR

ASN

ASP

PHE

9

GLY

ASN

TYR

ASN

GLN

SER

ASN

PHE

10

ASP

PRO

MET

LYS

GLY

ASN

PHE

LYS

ASP

11

ARG

SER

GLY

PRO

TYR

ASP

LYS

GLY

12

GLN

TYR

PHE

ALA

LYS

PRO

ARG

ASN

GLN

GLY

13

GLY

TYR

GLY

SER

LYS

SER

14

TYR

GLY

SER

ASP

ARG

PHE

Samples:

sample_1: +7K+12D hnRNPA1 LCD, [U-99% 13C; U-99% 15N], 157.5 uM; DSS 20 uM; D2O 10%; HEPES 20 mM; sodium chloride 150 mM

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D NH(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0 - collection

NMRViewJ v9.2 - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts