BMRB Entry 50740
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50740
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Title: Backbone assignments of the N-terminal region of the partitioning protein IncC1 from the plasmid RK2
Deposition date: 2021-01-29 Original release date: 2021-04-16
Authors: Ur-Rehman, Fayyaz; Jeeves, Mark; Hyde, Eva
Citation: Ur-Rehman, Fayyaz; Jeeves, Mark; Hyde, Eva. "Backbone assignments, and effect of Asn deamidation, of the N-terminal region of the partitioning protein IncC1 from the plasmid RK2" Biomol. NMR Assignments ., .-. (2021).
Assembly members:
entity_1, polymer, 128 residues, 13146 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pET28a
Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH
SEFMGVIHEETAYRKPVPGG
DPGAGSGAADHRDSAGRLSR
WEATGDVRNVAGTDQGRSVA
SGASRVGRVRGQELARGVRA
GNGGSAGTSGVHRPEVGSGR
QEKTGNQT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 354 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 121 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IncC1 NTD | 1 |
Entities:
Entity 1, IncC1 NTD 128 residues - 13146 Da.
-22M to 0F is the non-native affinity tag. The native sequence starts at 1M. This is the N-terminal of IncC1, which is not found in IncC2
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | SER | GLU | PHE | MET | GLY | VAL | ILE | HIS | GLU | GLU | ||||
| 4 | THR | ALA | TYR | ARG | LYS | PRO | VAL | PRO | GLY | GLY | ||||
| 5 | ASP | PRO | GLY | ALA | GLY | SER | GLY | ALA | ALA | ASP | ||||
| 6 | HIS | ARG | ASP | SER | ALA | GLY | ARG | LEU | SER | ARG | ||||
| 7 | TRP | GLU | ALA | THR | GLY | ASP | VAL | ARG | ASN | VAL | ||||
| 8 | ALA | GLY | THR | ASP | GLN | GLY | ARG | SER | VAL | ALA | ||||
| 9 | SER | GLY | ALA | SER | ARG | VAL | GLY | ARG | VAL | ARG | ||||
| 10 | GLY | GLN | GLU | LEU | ALA | ARG | GLY | VAL | ARG | ALA | ||||
| 11 | GLY | ASN | GLY | GLY | SER | ALA | GLY | THR | SER | GLY | ||||
| 12 | VAL | HIS | ARG | PRO | GLU | VAL | GLY | SER | GLY | ARG | ||||
| 13 | GLN | GLU | LYS | THR | GLY | ASN | GLN | THR |
Samples:
sample_1: IncC1 NTD, [U-100% 13C; U-100% 15N], 300 uM; D2O, [U-2H], 10%; H2O 90%; sodium phosphate 10 mM; sodium chloride 150 mM; EDTA 0.1 mM
sample_conditions_1: ionic strength: 0.173 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| CON | sample_1 | isotropic | sample_conditions_1 |
| (H)CBCACON | sample_1 | isotropic | sample_conditions_1 |
| (H)CBCACO | sample_1 | isotropic | sample_conditions_1 |
| HNN | sample_1 | isotropic | sample_conditions_1 |
| HNCN | sample_1 | isotropic | sample_conditions_1 |
| (H)CANCO | sample_1 | isotropic | sample_conditions_1 |
Software:
MddNMR - processing
NMRPipe - processing
ANALYSIS v2.0 - data analysis
TALOS-N - N Order parameter, secondary structure propensity
DANGLE - secondary structure propensity
xwinnmr - collection
NMR spectrometers:
- Bruker AVANCE III 600 MHz
- Bruker AVANCE III 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts