BMRB Entry 50743
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50743
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Title: dmEB1N+linker PubMed: 34215685
Deposition date: 2021-02-01 Original release date: 2021-03-01
Authors: Dimitrova-Paternoga, Lyudmila
Citation: Vaishali, Vaishali; Dimitrova-Paternoga, Lyudmila; Haubrich, Kevin; Sun, Mai; Ephrussi, Anne; Hennig, Janosch. "Validation and classification of RNA binding proteins identified by mRNA interactome capture" RNA 27, 1173-1185 (2021).
Assembly members:
entity_1, polymer, 209 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24d
Entity Sequences (FASTA):
entity_1: MAVNVYSTNVTSENLSRHDM
LAWVNDCLQSQFSKIEELCT
GAAYCQFMDMLFPNSVPVKR
VKFRTNLEHEYIQNFKILQA
GFKKMSVDKIIPIDKLVKGR
FQDNFEFLQWFKKFFDANYD
GRDYDASAVREGAPMGFGSG
AVKSLPGTAASGVSSSYRRG
PSATTRPAMTSAVKPTVSKV
LPRTNNAAPASRINACANST
GTVKKNDVS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 581 |
| 15N chemical shifts | 191 |
| 1H chemical shifts | 191 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EB1-N+Linker | 1 |
Entities:
Entity 1, EB1-N+Linker 209 residues - Formula weight is not available
| 1 | MET | ALA | VAL | ASN | VAL | TYR | SER | THR | ASN | VAL | ||||
| 2 | THR | SER | GLU | ASN | LEU | SER | ARG | HIS | ASP | MET | ||||
| 3 | LEU | ALA | TRP | VAL | ASN | ASP | CYS | LEU | GLN | SER | ||||
| 4 | GLN | PHE | SER | LYS | ILE | GLU | GLU | LEU | CYS | THR | ||||
| 5 | GLY | ALA | ALA | TYR | CYS | GLN | PHE | MET | ASP | MET | ||||
| 6 | LEU | PHE | PRO | ASN | SER | VAL | PRO | VAL | LYS | ARG | ||||
| 7 | VAL | LYS | PHE | ARG | THR | ASN | LEU | GLU | HIS | GLU | ||||
| 8 | TYR | ILE | GLN | ASN | PHE | LYS | ILE | LEU | GLN | ALA | ||||
| 9 | GLY | PHE | LYS | LYS | MET | SER | VAL | ASP | LYS | ILE | ||||
| 10 | ILE | PRO | ILE | ASP | LYS | LEU | VAL | LYS | GLY | ARG | ||||
| 11 | PHE | GLN | ASP | ASN | PHE | GLU | PHE | LEU | GLN | TRP | ||||
| 12 | PHE | LYS | LYS | PHE | PHE | ASP | ALA | ASN | TYR | ASP | ||||
| 13 | GLY | ARG | ASP | TYR | ASP | ALA | SER | ALA | VAL | ARG | ||||
| 14 | GLU | GLY | ALA | PRO | MET | GLY | PHE | GLY | SER | GLY | ||||
| 15 | ALA | VAL | LYS | SER | LEU | PRO | GLY | THR | ALA | ALA | ||||
| 16 | SER | GLY | VAL | SER | SER | SER | TYR | ARG | ARG | GLY | ||||
| 17 | PRO | SER | ALA | THR | THR | ARG | PRO | ALA | MET | THR | ||||
| 18 | SER | ALA | VAL | LYS | PRO | THR | VAL | SER | LYS | VAL | ||||
| 19 | LEU | PRO | ARG | THR | ASN | ASN | ALA | ALA | PRO | ALA | ||||
| 20 | SER | ARG | ILE | ASN | ALA | CYS | ALA | ASN | SER | THR | ||||
| 21 | GLY | THR | VAL | LYS | LYS | ASN | ASP | VAL | SER |
Samples:
sample_1: EB1-N+Linker, [U-100% 15N], 200 uM; PO4 20 mM; NaCl 150 mM; TCEP 0.5 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts