BMRB Entry 50744

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50744

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Title:
Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-p450-reductase enables high-resolution NMR probing
Deposition date:
2021-02-01
Original release date:
2021-05-18
Authors:
Krishnarjuna, Bankala; Ramamoorthy, Ayyalusamy
Citation:

Citation: Krishnarjuna, Bankala; Ramamoorthy, Ayyalusamy. "Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing"  Chem. Commun. (Camb) 57, 4819-4822 (2021).
PubMed: 33982687

Assembly members:

Assembly members:
entity_1, polymer, 239 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGDSHEDTSATMPEAVAEEV SLFSTTDMVLFSLIVGVLTY WFIFRKKKEEIPEFSKIQTT APPVKESSFVEKMKKTGRNI IVFYGSQTGTAEEFANRLSK DAHRYGMRGMSADPEEYDLA DLSSLPEIDKSLVVFCMATY GEGDPTDNAQDFYDWLQETD VDLTGVKFAVFGLGNKTYEH FNAMGKYVDQRLEQLGAQRI FELGLGDDDGNLEEDFITWR EQFWPAVCEFFGVEATGEE

Data sets:
Data typeCount
13C chemical shifts385
15N chemical shifts191
1H chemical shifts191

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein-lipid-nanodisc1

Entities:

Entity 1, Protein-lipid-nanodisc 239 residues - Formula weight is not available

1   METGLYASPSERHISGLUASPTHRSERALA
2   THRMETPROGLUALAVALALAGLUGLUVAL
3   SERLEUPHESERTHRTHRASPMETVALLEU
4   PHESERLEUILEVALGLYVALLEUTHRTYR
5   TRPPHEILEPHEARGLYSLYSLYSGLUGLU
6   ILEPROGLUPHESERLYSILEGLNTHRTHR
7   ALAPROPROVALLYSGLUSERSERPHEVAL
8   GLULYSMETLYSLYSTHRGLYARGASNILE
9   ILEVALPHETYRGLYSERGLNTHRGLYTHR
10   ALAGLUGLUPHEALAASNARGLEUSERLYS
11   ASPALAHISARGTYRGLYMETARGGLYMET
12   SERALAASPPROGLUGLUTYRASPLEUALA
13   ASPLEUSERSERLEUPROGLUILEASPLYS
14   SERLEUVALVALPHECYSMETALATHRTYR
15   GLYGLUGLYASPPROTHRASPASNALAGLN
16   ASPPHETYRASPTRPLEUGLNGLUTHRASP
17   VALASPLEUTHRGLYVALLYSPHEALAVAL
18   PHEGLYLEUGLYASNLYSTHRTYRGLUHIS
19   PHEASNALAMETGLYLYSTYRVALASPGLN
20   ARGLEUGLUGLNLEUGLYALAGLNARGILE
21   PHEGLULEUGLYLEUGLYASPASPASPGLY
22   ASNLEUGLUGLUASPPHEILETHRTRPARG
23   GLUGLNPHETRPPROALAVALCYSGLUPHE
24   PHEGLYVALGLUALATHRGLYGLUGLU

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SPARKY: Backbone or all simulated peaks