BMRB Entry 50752

Name: Backbone chemical shift assignments of PSD-95 N-terminal domain
Published: 2021-05-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50752

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone chemical shift assignments of PSD-95 N-terminal domain

Deposition date:

2021-02-08

Original release date:

2021-05-18

Authors:

Zhang, Yonghong

Citation:

Citation: Zhang, Yonghong; Hell, Johannes; Ames, James. "Chemical shift assignments of the N-terminal domain of PSD95 (PSD95-NT)" Biomolecular NMR Assignments 15, 347-350 (2021).
PubMed: 33929702

Assembly members:

Assembly members:
entity_1, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: E. coli Vector: pGEX-4T3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSMDCLCIVTTKKYRYQDED TPPLEHSPAHLPNQANSPPV IVNTDTLEAPGYELQVNGTE GEMEYEEITLERG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	197
15N chemical shifts	62
1H chemical shifts	256

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PSD-95NTD	1

Entities:

Entity 1, PSD-95NTD 73 residues - Formula weight is not available

1

GLY

SER

MET

ASP

CYS

LEU

CYS

ILE

VAL

THR

2

THR

LYS

TYR

ARG

TYR

GLN

ASP

GLU

ASP

3

THR

PRO

LEU

GLU

HIS

SER

PRO

ALA

HIS

4

LEU

PRO

ASN

GLN

ALA

ASN

SER

PRO

VAL

5

ILE

VAL

ASN

THR

ASP

THR

LEU

GLU

ALA

PRO

6

GLY

TYR

GLU

LEU

GLN

VAL

ASN

GLY

THR

GLU

7

GLY

GLU

MET

GLU

TYR

GLU

ILE

THR

LEU

8

GLU

ARG

GLY

Samples:

sample_1: PSD95NT-1, [U-99% 13C; U-99% 15N], 0.1 mM

sample_conditions_1: ionic strength: 36 mM; pH: 5; pressure: 1 atm; temperature: 290 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignments

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks