BMRB Entry 50754
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50754
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone and side-chain resonance assignments of the A2 domain of mouse von Willebrand factor PubMed: 34286417
Deposition date: 2021-02-08 Original release date: 2021-08-10
Authors: Morimoto, Daichi; Osugi, Masanori; Mahana, Yutaka; Walinda, Erik; Shirakawa, Masahiro; Sugase, Kenji
Citation: Morimoto, Daichi; Osugi, Masanori; Mahana, Yutaka; Walinda, Erik; Shirakawa, Masahiro; Sugase, Kenji. "Backbone resonance assignments of the A2 domain of mouse von Willebrand factor" Biomol. NMR Assignments 15, 427-431 (2021).
Assembly members:
entity_1, polymer, 182 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1
Entity Sequences (FASTA):
entity_1: GPLGSMVLDVVFVLEGSDEV
GEANFNKSKEFVEEVIQRMD
VSPDATRISVLQYSYTVTME
YAFNGAQSKEEVLRHVREIR
YQGGNRTNTGQALQYLSEHS
FSPSQGDRVEAPNLVYMVTG
NPASDEIKRLPGDIQVVPIG
VGPHANMQELERISRPIAPI
FIRDFETLPREAPDLVLQTC
CS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 519 |
| 15N chemical shifts | 163 |
| 1H chemical shifts | 163 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | vWF-A2 | 1 |
Entities:
Entity 1, vWF-A2 182 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | MET | VAL | LEU | ASP | VAL | ||||
| 2 | VAL | PHE | VAL | LEU | GLU | GLY | SER | ASP | GLU | VAL | ||||
| 3 | GLY | GLU | ALA | ASN | PHE | ASN | LYS | SER | LYS | GLU | ||||
| 4 | PHE | VAL | GLU | GLU | VAL | ILE | GLN | ARG | MET | ASP | ||||
| 5 | VAL | SER | PRO | ASP | ALA | THR | ARG | ILE | SER | VAL | ||||
| 6 | LEU | GLN | TYR | SER | TYR | THR | VAL | THR | MET | GLU | ||||
| 7 | TYR | ALA | PHE | ASN | GLY | ALA | GLN | SER | LYS | GLU | ||||
| 8 | GLU | VAL | LEU | ARG | HIS | VAL | ARG | GLU | ILE | ARG | ||||
| 9 | TYR | GLN | GLY | GLY | ASN | ARG | THR | ASN | THR | GLY | ||||
| 10 | GLN | ALA | LEU | GLN | TYR | LEU | SER | GLU | HIS | SER | ||||
| 11 | PHE | SER | PRO | SER | GLN | GLY | ASP | ARG | VAL | GLU | ||||
| 12 | ALA | PRO | ASN | LEU | VAL | TYR | MET | VAL | THR | GLY | ||||
| 13 | ASN | PRO | ALA | SER | ASP | GLU | ILE | LYS | ARG | LEU | ||||
| 14 | PRO | GLY | ASP | ILE | GLN | VAL | VAL | PRO | ILE | GLY | ||||
| 15 | VAL | GLY | PRO | HIS | ALA | ASN | MET | GLN | GLU | LEU | ||||
| 16 | GLU | ARG | ILE | SER | ARG | PRO | ILE | ALA | PRO | ILE | ||||
| 17 | PHE | ILE | ARG | ASP | PHE | GLU | THR | LEU | PRO | ARG | ||||
| 18 | GLU | ALA | PRO | ASP | LEU | VAL | LEU | GLN | THR | CYS | ||||
| 19 | CYS | SER |
Samples:
sample_1: vWF-A2, [U-99% 13C; U-99% 15N], 1.3 mM; Tris-HCl buffer 50 mM; calcium chloride 2 mM
sample_conditions_1: pH: 8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
MAGRO - chemical shift assignment
FLYA - chemical shift assignment
CcpNMR - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts