BMRB Entry 50758

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50758

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Title:
Backbone resonance assignment of C-terminal intracellular domain of Tir effector from Escherichia coli O127:H6
Deposition date:
2021-02-10
Original release date:
2024-02-14
Authors:
Vieira, Marta; Cordeiro, Tiago
Citation:

Citation: Vieira, Marta; Hernandez, Guillem; Zhong, Qiyun; Arbesu, Miguel; Veloso, Tiago; Gomes, Tiago; Martins, Maria; Monteiro, Hugo; Frazao, Carlos; Frankel, Gad; Zanzoni, Andreas; Cordeiro, Tiago. "The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection"  Commun. Biol. 7, 179-179 (2024).
PubMed: 38351154

Assembly members:

Assembly members:
entity_1, polymer, 173 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHTP8

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPRRNQPAEQTTTTTTHTVV QQQTGGNTPAQGGTDATRAE DASLNRRDSQGSVASTHWSD SSSEVVNPYAEVGGARNSLS AHQPEEHIYDEVAADPGYSV IQNFSGSGPVTGRLIGTPGQ GIQSTYALLANSGGLRLGMG GLTSGGESAVSSVNAAPTPG PVRFVWSHPQFEK

Data sets:
Data typeCount
13C chemical shifts494
15N chemical shifts160
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tir1

Entities:

Entity 1, Tir 173 residues - Formula weight is not available

N-terminal residues GP from cleavage with HRV-3C protease C-Terminal Streptag (residues WSHPQFEK)

1   GLYPROARGARGASNGLNPROALAGLUGLN
2   THRTHRTHRTHRTHRTHRHISTHRVALVAL
3   GLNGLNGLNTHRGLYGLYASNTHRPROALA
4   GLNGLYGLYTHRASPALATHRARGALAGLU
5   ASPALASERLEUASNARGARGASPSERGLN
6   GLYSERVALALASERTHRHISTRPSERASP
7   SERSERSERGLUVALVALASNPROTYRALA
8   GLUVALGLYGLYALAARGASNSERLEUSER
9   ALAHISGLNPROGLUGLUHISILETYRASP
10   GLUVALALAALAASPPROGLYTYRSERVAL
11   ILEGLNASNPHESERGLYSERGLYPROVAL
12   THRGLYARGLEUILEGLYTHRPROGLYGLN
13   GLYILEGLNSERTHRTYRALALEULEUALA
14   ASNSERGLYGLYLEUARGLEUGLYMETGLY
15   GLYLEUTHRSERGLYGLYGLUSERALAVAL
16   SERSERVALASNALAALAPROTHRPROGLY
17   PROVALARGPHEVALTRPSERHISPROGLN
18   PHEGLULYS

Related Database Links:

UNP B7UM99

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks