BMRB Entry 50766

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50766

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Title:
Backbone resonance assignments of LINE-1 retrotransposable element ORF1 protein N-terminal region.
Deposition date:
2021-02-16
Original release date:
2021-03-03
Authors:
Naik, Mandar; Fawzi, Nicolas; Jogl, Gerwald; Sedivy, John
Citation:

Citation: Newton, Jocelyn; Naik, Mandar; Li, Grace; Murphy, Eileen; Fawzi, Nicolas; Sedivy, John; Jogl, Gerwald. "Phase separation of the LINE-1 ORF1 protein is mediated by the N-terminus and coiled-coil domain."  Biophys. J. 120, 2181-2191 (2021).
PubMed: 33798566

Assembly members:

Assembly members:
entity_1, polymer, 61 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt26

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGKKQNRKTGNSKTQSASPP PKERSSSPATEQSWMENDFD ELREEGFRRSNYSLEHHHHH H

Data sets:
Data typeCount
13C chemical shifts170
15N chemical shifts54
1H chemical shifts54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ORF1 1-531

Entities:

Entity 1, ORF1 1-53 61 residues - Formula weight is not available

Residue 54 to 61 are non-native purification tag

1   METGLYLYSLYSGLNASNARGLYSTHRGLY
2   ASNSERLYSTHRGLNSERALASERPROPRO
3   PROLYSGLUARGSERSERSERPROALATHR
4   GLUGLNSERTRPMETGLUASNASPPHEASP
5   GLULEUARGGLUGLUGLYPHEARGARGSER
6   ASNTYRSERLEUGLUHISHISHISHISHIS
7   HIS

Related Database Links:

UNP Q9UN81
AlphaFold Q15605

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks