BMRB Entry 50776

Title:
Substrate sequence determinants governing the specificity of ClpS1 in plant chloroplasts
Deposition date:
2021-02-18
Original release date:
2021-06-14
Authors:
Aguilar Lucero, Dianela; Cantoia, Alejo; Sanchez-Lopez, Carolina; Binolfi, Andres; Mogk, Axel; Ceccarelli, Eduardo; Rosano, German
Citation:

Citation: Aguilar Lucero, Dianela; Cantoia, Alejo; Sanchez-Lopez, Carolina; Binolfi, Andres; Mogk, Axel; Ceccarelli, Eduardo; Rosano, German. "Structural features of the plant N-recognin ClpS1 and sequence determinants in its targets that govern substrate selection"  FEBS Lett. 595, 1525-1541 (2021).
PubMed: 33792910

Assembly members:

Assembly members:
entity_1, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETGEXCT

Data sets:
Data typeCount
13C chemical shifts339
15N chemical shifts105
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ClpS11

Entities:

Entity 1, ClpS1 123 residues - Formula weight is not available

First Ala residue comes from the cloning procedure and does not belong to the protein's original sequence.

1   ALAALAALALEUGLYLYSGLYGLYGLYVAL
2   LEUASPLYSPROILEILEGLULYSTHRTHR
3   PROGLYARGGLUSERGLUPHEASPLEUARG
4   LYSSERLYSLYSILEALAPROPROTYRARG
5   VALILELEUHISASNASPASNPHEASNLYS
6   ARGGLUTYRVALVALGLNVALLEUMETLYS
7   VALILEPROGLYMETTHRVALASPASNALA
8   VALASNILEMETGLNGLUALAHISILEASN
9   GLYLEUALAVALVALILEVALCYSALAGLN
10   ALAASPALAGLUGLNHISCYSMETGLNLEU
11   ARGGLYASNGLYLEULEUSERSERVALGLU
12   PROASPGLYGLYGLYCYSALAGLUPHELEU
13   VALPROARG

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