BMRB Entry 50789

Name: 1H, 13C and 15N chemical shift assignments of the C-terminal domain of human UDP-Glucuronosyltransferase 2B7 (UGT2B7)
Published: 2021-05-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50789

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift assignments of the C-terminal domain of human UDP-Glucuronosyltransferase 2B7 (UGT2B7)

Deposition date:

2021-02-24

Original release date:

2021-05-18

Authors:

Osborne, Michael; Rahardjo, Amanda; Volpon, Laurent; Borden, Katherine

Citation:

Citation: Osborne, Michael; Rahardjo, Amanda; Volpon, Laurent; Borden, Katherine. "1H, 13C and 15N chemical shift assignments of the C-terminal domain of human UDP-Glucuronosyltransferase 2B7 (UGT2B7-C)" Biomol. NMR Assignments 15, 323-328 (2021).
PubMed: 33870481

Assembly members:

Assembly members:
entity_1, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSAKPLPKEMEDFVQSSGEN GVVVFSLGSMVSNMTEERAN VIASALAQIPQKVLWRFDGN KPDTLGLNTRLYKWIPQNDL LGHPKTRAFITHGGANGIYE AIYHGIPMVGIPLFADQPDN IAHMKARGAAVRVDFNTMSS TDLLNALKRVINDPSYKENV MKLSRIQHD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	606
15N chemical shifts	145
1H chemical shifts	825

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	c-terminus of UGT2B7	1

Entities:

Entity 1, c-terminus of UGT2B7 169 residues - Formula weight is not available

1

GLY

SER

ALA

LYS

PRO

LEU

PRO

LYS

GLU

MET

2

GLU

ASP

PHE

VAL

GLN

SER

GLY

GLU

ASN

3

GLY

VAL

PHE

SER

LEU

GLY

SER

MET

4

VAL

SER

ASN

MET

THR

GLU

ARG

ALA

ASN

5

VAL

ILE

ALA

SER

ALA

LEU

ALA

GLN

ILE

PRO

6

GLN

LYS

VAL

LEU

TRP

ARG

PHE

ASP

GLY

ASN

7

LYS

PRO

ASP

THR

LEU

GLY

LEU

ASN

THR

ARG

8

LEU

TYR

LYS

TRP

ILE

PRO

GLN

ASN

ASP

LEU

9

LEU

GLY

HIS

PRO

LYS

THR

ARG

ALA

PHE

ILE

10

THR

HIS

GLY

ALA

ASN

GLY

ILE

TYR

GLU

11

ALA

ILE

TYR

HIS

GLY

ILE

PRO

MET

VAL

GLY

12

ILE

PRO

LEU

PHE

ALA

ASP

GLN

PRO

ASP

ASN

13

ILE

ALA

HIS

MET

LYS

ALA

ARG

GLY

ALA

14

VAL

ARG

VAL

ASP

PHE

ASN

THR

MET

SER

15

THR

ASP

LEU

ASN

ALA

LEU

LYS

ARG

VAL

16

ILE

ASN

ASP

PRO

SER

TYR

LYS

GLU

ASN

VAL

17

MET

LYS

LEU

SER

ARG

ILE

GLN

HIS

ASP

Samples:

sample_1: C- terminus of UGT2B7, [U-100% 13C; U-100% 15N], 300 uM; sodium phosphate 50 mM; sodium chloride 300 mM; DTT 1 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.3 M; pH: 7.2; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRViewJ - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz