BMRB Entry 50798
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50798
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Title: GHR-TMD in DHPC micelles PubMed: 34193419
Deposition date: 2021-03-02 Original release date: 2021-08-10
Authors: Steinocher, Helena; Bugge, Katrine; Kragelund, Birthe
Citation: Kassem, Noah; Araya-Secchi, Raul; Bugge, Katrine; Barclay, Abigail; Steinocher, Helena; Khondker, Adree; Wang, Yong; J. Lenard, Aneta; Burck, Jochen; Sahin, Cagla; S. Ulrich, Anne; Landreh, Michael; Cramer Pedersen, Martin; C. Rheinstadter, Maikel; Amstrup Pedersen, Per; Lindorff-Larsen, Kresten; Arleth, Lise; B. Kragelund, Birthe. "Order and disorder-An integrative structure of the full-length human growth hormone receptor" Sci. Adv. 7, eabh3805-eabh3805 (2021).
Assembly members:
entity_1, polymer, 39 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX
Entity Sequences (FASTA):
entity_1: GSQFTCEEDFYFPWLLIIIF
GIFGLTVMLFVFLFSKQQR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 101 |
| 15N chemical shifts | 35 |
| 1H chemical shifts | 52 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TMD | 1 |
Entities:
Entity 1, TMD 39 residues - Formula weight is not available
| 1 | GLY | SER | GLN | PHE | THR | CYS | GLU | GLU | ASP | PHE | ||||
| 2 | TYR | PHE | PRO | TRP | LEU | LEU | ILE | ILE | ILE | PHE | ||||
| 3 | GLY | ILE | PHE | GLY | LEU | THR | VAL | MET | LEU | PHE | ||||
| 4 | VAL | PHE | LEU | PHE | SER | LYS | GLN | GLN | ARG |
Samples:
sample_1: GHR-TMD, [U-13C; U-15N], 1 mM; DSS 1 mM; TCEP 2 mM; sodium azide 0.05 % v/v; potassium chloride 50 mM; sodium phosphate 20 mM; DHPC 210 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCAHC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker Avance 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts