BMRB Entry 50816
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50816
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Title: 1H, 13C and 15N Backbone Chemical Shift Assignments of the V517A SH3 variant of the JNK-interacting protein 1 PubMed: 35173330
Deposition date: 2021-03-10 Original release date: 2021-12-13
Authors: Marino Perez, Laura; Ringkjobing Jensen, Malene
Citation: Marino Perez, Laura; Ielasi, Francesco; Mamigonian Bessa, Luiza; Maurin, Damien; Kragelj, Jaka; Blackledge, Martin; Salvi, Nicola; Bouvignies, Guillaume; Palencia, Andres; Jensen, Malene. "Visualizing protein breathing motions associated with aromatic ring flipping" Nature 602, 695-700 (2022).
Assembly members:
entity_1, polymer, 63 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: GHMEQTHRAIFRFVPRHEDE
LELEVDDPLLAELQAEDYWY
EAYNMRTGARGVFPAYYAIE
VTK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 120 |
15N chemical shifts | 56 |
1H chemical shifts | 56 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | JIP1-SH3-V517A | 1 |
Entities:
Entity 1, JIP1-SH3-V517A 63 residues - Formula weight is not available
1 | GLY | HIS | MET | GLU | GLN | THR | HIS | ARG | ALA | ILE | ||||
2 | PHE | ARG | PHE | VAL | PRO | ARG | HIS | GLU | ASP | GLU | ||||
3 | LEU | GLU | LEU | GLU | VAL | ASP | ASP | PRO | LEU | LEU | ||||
4 | ALA | GLU | LEU | GLN | ALA | GLU | ASP | TYR | TRP | TYR | ||||
5 | GLU | ALA | TYR | ASN | MET | ARG | THR | GLY | ALA | ARG | ||||
6 | GLY | VAL | PHE | PRO | ALA | TYR | TYR | ALA | ILE | GLU | ||||
7 | VAL | THR | LYS |
Samples:
sample_1: JIP1-SH3-V517A, [U-95% 13C; U-95% 15N], 2.0 mM; NaCl 150 mM; HEPES 50 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 308 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking
TOPSPIN - collection
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts