BMRB Entry 50842

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PDB ID: 7mc1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50842
MolProbity Validation Chart

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Title:
Solution structure of Miz-1 Zinc fingers 10 to 12
Deposition date:
2021-03-23
Original release date:
2021-04-08
Authors:
Boisvert, Olivier; Lavigne, Pierre
Citation:

Citation: Boisvert, Olivier; Letourneau, Danny; Delattre, Patrick; Tremblay, Cynthia; Jolibois, Emilie; Montagne, Martin; Lavigne, Pierre. "Zinc Fingers 10 and 11 of Miz-1 undergo conformational exchange to achieve specific DNA binding"  Structure 30, 623-636 (2022).
PubMed: 34963061

Assembly members:

Assembly members:
entity_1, polymer, 85 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKPYVCERCGKRFVQSSQLA NHIRHHDNIRPYKCSVCSKA FVNVGDLSKHIIIHTGEKPY LCDKCGRGFNRVDNLRSHVK TVHQG

Data typeCount
13C chemical shifts327
15N chemical shifts82
1H chemical shifts497

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1miz-1 10-121
2Zinc, 12
3Zinc, 22
4Zinc, 32

Entities:

Entity 1, miz-1 10-12 85 residues - Formula weight is not available

1   METLYSPROTYRVALCYSGLUARGCYSGLY
2   LYSARGPHEVALGLNSERSERGLNLEUALA
3   ASNHISILEARGHISHISASPASNILEARG
4   PROTYRLYSCYSSERVALCYSSERLYSALA
5   PHEVALASNVALGLYASPLEUSERLYSHIS
6   ILEILEILEHISTHRGLYGLULYSPROTYR
7   LEUCYSASPLYSCYSGLYARGGLYPHEASN
8   ARGVALASPASNLEUARGSERHISVALLYS
9   THRVALHISGLNGLY

Entity 2, Zinc, 1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

UNP Q13105
AlphaFold Q9NUC9 Q9NUC9 Q9NUC9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks