BMRB Entry 50848
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50848
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: methylated ZEBRA-responsive element 2 DNA PubMed: 34893887
Deposition date: 2021-03-25 Original release date: 2021-11-24
Authors: Gunther, Johannes
Citation: Bernaudat, Florent; Gustems, Montse; Gunther, Johannes; Oliva, Mizar; Buschle, Alexander; Gobel, Christine; Pagniez, Priscilla; Lupo, Julien; Signor, Luca; Muller, Christoph; Morand, Patrice; Sattler, Michael; Hammerschmidt, Wolfgang; Petosa, Carlo. "Structural basis of DNA methylation-dependent site selectivity of the Epstein-Barr virus lytic switch protein ZEBRA/Zta/BZLF1" Nucleic Acids Res. 50, 490-511 (2022).
Assembly members:
entity_1, polymer, 18 residues, Formula weight is not available
entity_2, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: Human gammaherpesvirus 4 Taxonomy ID: 10376 Superkingdom: Viruses Kingdom: not available Genus/species: Lymphocryptovirus Epstein Barr Virus
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
entity_1: GCTTATGAGXGATTTTAT
entity_2: CGAATACTCGXTAAAATA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Chain A | 1 |
| 2 | Chain B | 2 |
Entities:
Entity 1, Chain A 18 residues - Formula weight is not available
| 1 | DG | DC | DT | DT | DA | DT | DG | DA | DG | 5MC | ||||
| 2 | DG | DA | DT | DT | DT | DT | DA | DT |
Entity 2, Chain B 18 residues - Formula weight is not available
| 1 | DC | DG | DA | DA | DT | DA | DC | DT | DC | DG | ||||
| 2 | 5MC | DT | DA | DA | DA | DA | DT | DA |
Samples:
sample_1: methylated ZEBRA-responsive element 2 DNA (ZRE2) 400 uM; sodium phosphate 50 mM; sodium chloride 100 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4.2 - chemical shift assignment and data analysis
TOPSPIN v3.5 pl6 - processing
NMR spectrometers:
- Bruker AVANCE III HD 950 MHz