BMRB Entry 50860

Name: Tim9 reduced
Published: 2021-04-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50860

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Tim9 reduced PubMed: 33974880

Deposition date: 2021-03-27 Original release date: 2021-04-22

Authors: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul

Citation: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul. "Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9*10*12" Structure 29, 1065-1073 (2021).

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet

Entity Sequences (FASTA):
entity_1: MDALNSKEQQEFQKVVEQKQ MKDFMRLYSNLVERCFTDCV NDFTTSKLTNKEQTCIMKCS EKFLKHSERVGQRFQEQNAA LGQGLGR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	217
15N chemical shifts	75
1H chemical shifts	75

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tim9 reduced	1

Entities:

Entity 1, Tim9 reduced 87 residues - Formula weight is not available

1

MET

ASP

ALA

LEU

ASN

SER

LYS

GLU

GLN

2

GLU

PHE

GLN

LYS

VAL

GLU

GLN

LYS

GLN

3

MET

LYS

ASP

PHE

MET

ARG

LEU

TYR

SER

ASN

4

LEU

VAL

GLU

ARG

CYS

PHE

THR

ASP

CYS

VAL

5

ASN

ASP

PHE

THR

SER

LYS

LEU

THR

ASN

6

LYS

GLU

GLN

THR

CYS

ILE

MET

LYS

CYS

SER

7

GLU

LYS

PHE

LEU

LYS

HIS

SER

GLU

ARG

VAL

8

GLY

GLN

ARG

PHE

GLN

GLU

GLN

ASN

ALA

9

LEU

GLY

GLN

GLY

LEU

GLY

ARG

Samples:

sample_1: Tim9, [U-13C; U-15N], 0.4 mM; MES 20 mM; sodium chloride 50 mM; TCEP 10 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts