BMRB Entry 50862
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50862
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Title: Tim10 PubMed: 33974880
Deposition date: 2021-03-27 Original release date: 2021-04-22
Authors: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul
Citation: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul. "Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9*10*12" Structure 29, 1065-1073 (2021).
Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet
Entity Sequences (FASTA):
entity_1: GSFLGFGGGQPQLSSQQKIQ
AAEAELDLVTDMFNKLVNNC
YKKCINTSYSEGELNKNESS
CLDRCVAKYFETNVQVGENM
QKMGQSFNAAGKF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 228 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 78 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tim10 reduced | 1 |
Entities:
Entity 1, Tim10 reduced 93 residues - Formula weight is not available
| 1 | GLY | SER | PHE | LEU | GLY | PHE | GLY | GLY | GLY | GLN | ||||
| 2 | PRO | GLN | LEU | SER | SER | GLN | GLN | LYS | ILE | GLN | ||||
| 3 | ALA | ALA | GLU | ALA | GLU | LEU | ASP | LEU | VAL | THR | ||||
| 4 | ASP | MET | PHE | ASN | LYS | LEU | VAL | ASN | ASN | CYS | ||||
| 5 | TYR | LYS | LYS | CYS | ILE | ASN | THR | SER | TYR | SER | ||||
| 6 | GLU | GLY | GLU | LEU | ASN | LYS | ASN | GLU | SER | SER | ||||
| 7 | CYS | LEU | ASP | ARG | CYS | VAL | ALA | LYS | TYR | PHE | ||||
| 8 | GLU | THR | ASN | VAL | GLN | VAL | GLY | GLU | ASN | MET | ||||
| 9 | GLN | LYS | MET | GLY | GLN | SER | PHE | ASN | ALA | ALA | ||||
| 10 | GLY | LYS | PHE |
Samples:
sample_1: Tim10 reduced, [U-13C; U-15N], 0.4 mM; MES 20 mM; sodium chloride 50 mM; TCEP 10 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K
sample_conditions_2: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_2 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_2 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_2 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_2 |
Software:
ANALYSIS - chemical shift assignment
NMRPipe - processing
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts