BMRB Entry 50864
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50864
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Title: Tim12 PubMed: 33974880
Deposition date: 2021-03-27 Original release date: 2021-04-22
Authors: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul
Citation: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul. "Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9*10*12" Structure 29, 1065-1073 (2021).
Assembly members:
entity_1, polymer, 109 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: GSFFLNSLRGNQEVSQEKLD
VAGVQFDAMASTFNNILSTC
LEKCIPHEGFGEPDLTKGEQ
ACIDRCVAKMHYSNRLIGGF
VQTRGFGPENQLRHYSRFVA
KEIADDSKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 223 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tim12 | 1 |
Entities:
Entity 1, Tim12 109 residues - Formula weight is not available
| 1 | GLY | SER | PHE | PHE | LEU | ASN | SER | LEU | ARG | GLY | ||||
| 2 | ASN | GLN | GLU | VAL | SER | GLN | GLU | LYS | LEU | ASP | ||||
| 3 | VAL | ALA | GLY | VAL | GLN | PHE | ASP | ALA | MET | ALA | ||||
| 4 | SER | THR | PHE | ASN | ASN | ILE | LEU | SER | THR | CYS | ||||
| 5 | LEU | GLU | LYS | CYS | ILE | PRO | HIS | GLU | GLY | PHE | ||||
| 6 | GLY | GLU | PRO | ASP | LEU | THR | LYS | GLY | GLU | GLN | ||||
| 7 | ALA | CYS | ILE | ASP | ARG | CYS | VAL | ALA | LYS | MET | ||||
| 8 | HIS | TYR | SER | ASN | ARG | LEU | ILE | GLY | GLY | PHE | ||||
| 9 | VAL | GLN | THR | ARG | GLY | PHE | GLY | PRO | GLU | ASN | ||||
| 10 | GLN | LEU | ARG | HIS | TYR | SER | ARG | PHE | VAL | ALA | ||||
| 11 | LYS | GLU | ILE | ALA | ASP | ASP | SER | LYS | LYS |
Samples:
sample_1: Tim12 reduced, [U-13C; U-15N], 0.2 mM; MES 20 mM; sodium chloride 50 mM; TCEP 10 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
Software:
ANALYSIS - chemical shift assignment
NMRPipe - processing
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts