BMRB Entry 50866
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50866
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Title: CITED2-HIF-1alpha fusion peptide PubMed: 34520739
Deposition date: 2021-03-31 Original release date: 2021-07-24
Authors: Appling, Francis; Berlow, Rebecca; Stanfield, Robyn; Dyson, H Jane; Wright, Peter
Citation: Appling, Francis; Berlow, Rebecca; Stanfield, Robyn; Dyson, H Jane; Wright, Peter. "The molecular basis of allostery in a facilitated dissociation process" Structure 29, 1327-1338 (2021).
Assembly members:
entity_1, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b
Entity Sequences (FASTA):
entity_1: GSHMSNVIDTDFIDEEVLMS
LVIEMGLDRIKELPELTSYD
CEVNAPIQGSRNLLQGEELL
RALDQVN
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 188 |
15N chemical shifts | 61 |
1H chemical shifts | 61 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | CITED2-HIF-1alpha fusion peptide | 1 |
Entities:
Entity 1, CITED2-HIF-1alpha fusion peptide 67 residues - Formula weight is not available
1 | GLY | SER | HIS | MET | SER | ASN | VAL | ILE | ASP | THR | ||||
2 | ASP | PHE | ILE | ASP | GLU | GLU | VAL | LEU | MET | SER | ||||
3 | LEU | VAL | ILE | GLU | MET | GLY | LEU | ASP | ARG | ILE | ||||
4 | LYS | GLU | LEU | PRO | GLU | LEU | THR | SER | TYR | ASP | ||||
5 | CYS | GLU | VAL | ASN | ALA | PRO | ILE | GLN | GLY | SER | ||||
6 | ARG | ASN | LEU | LEU | GLN | GLY | GLU | GLU | LEU | LEU | ||||
7 | ARG | ALA | LEU | ASP | GLN | VAL | ASN |
Samples:
sample_1: CITED2-HIF-1alpha fusion peptide, [U-100% 13C; U-100% 15N], 100 uM; NaCl 50 mM; DTT 2 mM; TRIS 20 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D best-HNCO | sample_1 | isotropic | sample_conditions_1 |
3D best-HNCA | sample_1 | isotropic | sample_conditions_1 |
3D best-HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D best-HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D best-HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRbox - data analysis, processing
GNU Octave - data analysis
NMRFAM-SPARKY - data analysis
NMRPipe - processing
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts