BMRB Entry 50890
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50890
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Title: Backbone NMR assignments of the mitochondrial membrane protein MPV17 without cysteine residues PubMed: 34116124
Deposition date: 2021-04-14 Original release date: 2021-06-25
Authors: Sperl, Laura; Hagn, Franz
Citation: Sperl, Laura; Hagn, Franz. "NMR Structural and Biophysical Analysis of the Disease-Linked Inner Mitochondrial Membrane Protein MPV17." J. Mol. Biol. 433, 167098-167098 (2021).
Assembly members:
entity_1, polymer, 176 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b-GB1
Entity Sequences (FASTA):
entity_1: MALWRAYQRALAAHPWKVQV
LTAGSLMGLGDIISQQLVER
RGLQEHQRGRTLTMVSLGAG
FVGPVVGGWYKVLDRFIPGT
TKVDALKKMLLDQGGFAPAF
LGAFLPLVGALNGLSAQDNW
AKLQRDYPDALITNYYLWPA
VQLANFYLVPLHYRLAVVQA
VAVIWNSYLSWKAHRL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 454 |
| 15N chemical shifts | 149 |
| 1H chemical shifts | 149 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MPV17 | 1 |
Entities:
Entity 1, MPV17 176 residues - Formula weight is not available
| 1 | MET | ALA | LEU | TRP | ARG | ALA | TYR | GLN | ARG | ALA | ||||
| 2 | LEU | ALA | ALA | HIS | PRO | TRP | LYS | VAL | GLN | VAL | ||||
| 3 | LEU | THR | ALA | GLY | SER | LEU | MET | GLY | LEU | GLY | ||||
| 4 | ASP | ILE | ILE | SER | GLN | GLN | LEU | VAL | GLU | ARG | ||||
| 5 | ARG | GLY | LEU | GLN | GLU | HIS | GLN | ARG | GLY | ARG | ||||
| 6 | THR | LEU | THR | MET | VAL | SER | LEU | GLY | ALA | GLY | ||||
| 7 | PHE | VAL | GLY | PRO | VAL | VAL | GLY | GLY | TRP | TYR | ||||
| 8 | LYS | VAL | LEU | ASP | ARG | PHE | ILE | PRO | GLY | THR | ||||
| 9 | THR | LYS | VAL | ASP | ALA | LEU | LYS | LYS | MET | LEU | ||||
| 10 | LEU | ASP | GLN | GLY | GLY | PHE | ALA | PRO | ALA | PHE | ||||
| 11 | LEU | GLY | ALA | PHE | LEU | PRO | LEU | VAL | GLY | ALA | ||||
| 12 | LEU | ASN | GLY | LEU | SER | ALA | GLN | ASP | ASN | TRP | ||||
| 13 | ALA | LYS | LEU | GLN | ARG | ASP | TYR | PRO | ASP | ALA | ||||
| 14 | LEU | ILE | THR | ASN | TYR | TYR | LEU | TRP | PRO | ALA | ||||
| 15 | VAL | GLN | LEU | ALA | ASN | PHE | TYR | LEU | VAL | PRO | ||||
| 16 | LEU | HIS | TYR | ARG | LEU | ALA | VAL | VAL | GLN | ALA | ||||
| 17 | VAL | ALA | VAL | ILE | TRP | ASN | SER | TYR | LEU | SER | ||||
| 18 | TRP | LYS | ALA | HIS | ARG | LEU |
Samples:
sample_1: MPV17 cysfree, [U-13C; U-15N; U-2H], 400 uM; NaPi 20 mM; NaCl 50 mM; EDTA 0.5 mM; DTT 5 mM; DPC 300 mM
sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection, processing
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts