BMRB Entry 50903
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50903
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Title: TCPTP residues 1-302 PubMed: 35013194
Deposition date: 2021-04-20 Original release date: 2021-11-10
Authors: Singh, Jai; Li, Yang; Peti, Wolfgang; Meng, Tzu-Ching; Page, Rebecca
Citation: Singh, Jai Prakash; Li, Yang; Chen, Yi-Yun; Hsu, Shang-Te Danny; Page, Rebecca; Peti, Wolfgang; Meng, Tzu-Ching. "The catalytic activity of TCPTP is auto-regulated by its intrinsically disordered tail and activated by Integrin alpha-1" Nat. Commun. 13, 94-94 (2022).
Assembly members:
entity_1, polymer, 307 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRP1B
Entity Sequences (FASTA):
entity_1: GHMGSMPTTIEREFEELDTQ
RRWQPLYLEIRNESHDYPHR
VAKFPENRNRNRYRDVSPYD
HSRVKLQNAENDYINASLVD
IEEAQRSYILTQGPLPNTCC
HFWLMVWQQKTKAVVMLNRI
VEKESVKCAQYWPTDDQEML
FKETGFSVKLLSEDVKSYYT
VHLLQLENINSGETRTISHF
HYTTWPDFGVPESPASFLNF
LFKVRESGSLNPDHGPAVIH
CSAGIGRSGTFSLVDTCLVL
MEKGDDINIKQVLLNMRKYR
MGLIQTPDQLRFSYMAIIEG
AKCIKGDSSIQKRWKELSKE
DLSPAFD
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 602 |
15N chemical shifts | 211 |
1H chemical shifts | 211 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | TCPTP 1-302 | 1 |
Entities:
Entity 1, TCPTP 1-302 307 residues - Formula weight is not available
The first five residues GHMGS are from cloning vector, TCPTP sequence starts from MPTTI.
1 | GLY | HIS | MET | GLY | SER | MET | PRO | THR | THR | ILE | ||||
2 | GLU | ARG | GLU | PHE | GLU | GLU | LEU | ASP | THR | GLN | ||||
3 | ARG | ARG | TRP | GLN | PRO | LEU | TYR | LEU | GLU | ILE | ||||
4 | ARG | ASN | GLU | SER | HIS | ASP | TYR | PRO | HIS | ARG | ||||
5 | VAL | ALA | LYS | PHE | PRO | GLU | ASN | ARG | ASN | ARG | ||||
6 | ASN | ARG | TYR | ARG | ASP | VAL | SER | PRO | TYR | ASP | ||||
7 | HIS | SER | ARG | VAL | LYS | LEU | GLN | ASN | ALA | GLU | ||||
8 | ASN | ASP | TYR | ILE | ASN | ALA | SER | LEU | VAL | ASP | ||||
9 | ILE | GLU | GLU | ALA | GLN | ARG | SER | TYR | ILE | LEU | ||||
10 | THR | GLN | GLY | PRO | LEU | PRO | ASN | THR | CYS | CYS | ||||
11 | HIS | PHE | TRP | LEU | MET | VAL | TRP | GLN | GLN | LYS | ||||
12 | THR | LYS | ALA | VAL | VAL | MET | LEU | ASN | ARG | ILE | ||||
13 | VAL | GLU | LYS | GLU | SER | VAL | LYS | CYS | ALA | GLN | ||||
14 | TYR | TRP | PRO | THR | ASP | ASP | GLN | GLU | MET | LEU | ||||
15 | PHE | LYS | GLU | THR | GLY | PHE | SER | VAL | LYS | LEU | ||||
16 | LEU | SER | GLU | ASP | VAL | LYS | SER | TYR | TYR | THR | ||||
17 | VAL | HIS | LEU | LEU | GLN | LEU | GLU | ASN | ILE | ASN | ||||
18 | SER | GLY | GLU | THR | ARG | THR | ILE | SER | HIS | PHE | ||||
19 | HIS | TYR | THR | THR | TRP | PRO | ASP | PHE | GLY | VAL | ||||
20 | PRO | GLU | SER | PRO | ALA | SER | PHE | LEU | ASN | PHE | ||||
21 | LEU | PHE | LYS | VAL | ARG | GLU | SER | GLY | SER | LEU | ||||
22 | ASN | PRO | ASP | HIS | GLY | PRO | ALA | VAL | ILE | HIS | ||||
23 | CYS | SER | ALA | GLY | ILE | GLY | ARG | SER | GLY | THR | ||||
24 | PHE | SER | LEU | VAL | ASP | THR | CYS | LEU | VAL | LEU | ||||
25 | MET | GLU | LYS | GLY | ASP | ASP | ILE | ASN | ILE | LYS | ||||
26 | GLN | VAL | LEU | LEU | ASN | MET | ARG | LYS | TYR | ARG | ||||
27 | MET | GLY | LEU | ILE | GLN | THR | PRO | ASP | GLN | LEU | ||||
28 | ARG | PHE | SER | TYR | MET | ALA | ILE | ILE | GLU | GLY | ||||
29 | ALA | LYS | CYS | ILE | LYS | GLY | ASP | SER | SER | ILE | ||||
30 | GLN | LYS | ARG | TRP | LYS | GLU | LEU | SER | LYS | GLU | ||||
31 | ASP | LEU | SER | PRO | ALA | PHE | ASP |
Samples:
sample_1: TCPTP residues 1-302, [U-100% 13C; U-100% 15N; U-80% 2H], 450 uM; HEPES 20 mM; sodium chloride 150 mM; TCEP 0.5 mM
sample_conditions_1: ionic strength: 170 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection, processing
CARA - chemical shift assignment
CcpNMR - data analysis
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts