BMRB Entry 50906
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50906
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Title: Chemical shifts of human Dss1 PubMed: 34272906
Deposition date: 2021-04-22 Original release date: 2021-08-16
Authors: Ruidiaz, Sarah; Dreier, Jesper; Hartmann-Petersen, Rasmus; Kragelund, Birthe
Citation: Ruidiaz, Sarah; Dreier, Jesper; Hartmann-Petersen, Rasmus; Kragelund, Birthe. "The disordered PCI-binding human proteins CSNAP and DSS1 have diverged in structure and function" Protein Sci. 30, 2069-2082 (2021).
Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pD454-GST
Entity Sequences (FASTA):
entity_1: GMSEKKQPVDLGLLEEDDEF
EEFPAEDWAGLDEDEDAHVW
EDNWDDDNVEDDFSNQLRAE
LEKHGYKMETS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 207 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 67 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Dss1 | 1 |
Entities:
Entity 1, Dss1 71 residues - Formula weight is not available
Non-native N-terminal Glycine
| 1 | GLY | MET | SER | GLU | LYS | LYS | GLN | PRO | VAL | ASP | ||||
| 2 | LEU | GLY | LEU | LEU | GLU | GLU | ASP | ASP | GLU | PHE | ||||
| 3 | GLU | GLU | PHE | PRO | ALA | GLU | ASP | TRP | ALA | GLY | ||||
| 4 | LEU | ASP | GLU | ASP | GLU | ASP | ALA | HIS | VAL | TRP | ||||
| 5 | GLU | ASP | ASN | TRP | ASP | ASP | ASP | ASN | VAL | GLU | ||||
| 6 | ASP | ASP | PHE | SER | ASN | GLN | LEU | ARG | ALA | GLU | ||||
| 7 | LEU | GLU | LYS | HIS | GLY | TYR | LYS | MET | GLU | THR | ||||
| 8 | SER |
Samples:
sample_1: DSS1, [U-100% 13C; U-100% 15N], 140 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DSS 1%; sodium azide 1%
sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)NNH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts