BMRB Entry 50908
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50908
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: human CSNAP PubMed: 34272906
Deposition date: 2021-04-22 Original release date: 2021-08-16
Authors: Ruidiaz, Sarah; Dreier, Jesper; Hartmann-Petersen, Rasmus; Kragelund, Birthe
Citation: Ruidiaz, Sarah; Dreier, Jesper; Hartmann-Petersen, Rasmus; Kragelund, Birthe. "The disordered PCI-binding human proteins CSNAP and DSS1 have diverged in structure and function" Protein Sci. 30, 2069-2082 (2021).
Assembly members:
entity_1, polymer, 58 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pD454-GST
Entity Sequences (FASTA):
entity_1: GMKPAVDEMFPEGAGPYVDL
DEAGGSTGLLMDLAANEKAV
HADFFNDFEDLFDDDDIQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 166 |
| 15N chemical shifts | 53 |
| 1H chemical shifts | 53 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CSNAP | 1 |
Entities:
Entity 1, CSNAP 58 residues - Formula weight is not available
| 1 | GLY | MET | LYS | PRO | ALA | VAL | ASP | GLU | MET | PHE | ||||
| 2 | PRO | GLU | GLY | ALA | GLY | PRO | TYR | VAL | ASP | LEU | ||||
| 3 | ASP | GLU | ALA | GLY | GLY | SER | THR | GLY | LEU | LEU | ||||
| 4 | MET | ASP | LEU | ALA | ALA | ASN | GLU | LYS | ALA | VAL | ||||
| 5 | HIS | ALA | ASP | PHE | PHE | ASN | ASP | PHE | GLU | ASP | ||||
| 6 | LEU | PHE | ASP | ASP | ASP | ASP | ILE | GLN |
Samples:
sample_1: CSNAP, [U-100% 13C; U-100% 15N], 210 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DSS 1%; sodium azide 1%
sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)NNH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Agilent DD2 800 MHz
- Bruker AVANCE III 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts