BMRB Entry 50914
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50914
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Title: Backbone (NH, CA, CB) chemical shifts of free ERH-2 from C.elegans PubMed: 34413138
Deposition date: 2021-04-28 Original release date: 2021-08-28
Authors: Perez-Borrajero, Cecilia; Hennig, Janosch
Citation: Perez-Borrajero, Cecilia; Podvalnaya, Nadezda; Holleis, Kay; Lichtenberger, Raffael; Karaulanov, Emil; Simon, Bernd; Basquin, Jerome; Hennig, Janosch; Ketting, Rene; Falk, Sebastian. "Structural basis of PETISCO complex assembly during piRNA biogenesis in C. elegans" Genes Dev. 35, 1304-1323 (2021).
Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available
Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: GHMASMSTSSHTVLLIQTSP
RLDSRTWGDYESVTDALDAL
CKMFEDFLSKKSAAPVTYDV
SQVYEFLDKLSDVSMMIFNR
ETGQYIGRTRAWIKQQVYEM
MRGRCQHPEGGEKVIVGY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 191 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 99 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ERH-2, chain 1 | 1 |
| 2 | ERH-2, chain 2 | 1 |
Entities:
Entity 1, ERH-2, chain 1 118 residues - Formula weight is not available
| 1 | GLY | HIS | MET | ALA | SER | MET | SER | THR | SER | SER | ||||
| 2 | HIS | THR | VAL | LEU | LEU | ILE | GLN | THR | SER | PRO | ||||
| 3 | ARG | LEU | ASP | SER | ARG | THR | TRP | GLY | ASP | TYR | ||||
| 4 | GLU | SER | VAL | THR | ASP | ALA | LEU | ASP | ALA | LEU | ||||
| 5 | CYS | LYS | MET | PHE | GLU | ASP | PHE | LEU | SER | LYS | ||||
| 6 | LYS | SER | ALA | ALA | PRO | VAL | THR | TYR | ASP | VAL | ||||
| 7 | SER | GLN | VAL | TYR | GLU | PHE | LEU | ASP | LYS | LEU | ||||
| 8 | SER | ASP | VAL | SER | MET | MET | ILE | PHE | ASN | ARG | ||||
| 9 | GLU | THR | GLY | GLN | TYR | ILE | GLY | ARG | THR | ARG | ||||
| 10 | ALA | TRP | ILE | LYS | GLN | GLN | VAL | TYR | GLU | MET | ||||
| 11 | MET | ARG | GLY | ARG | CYS | GLN | HIS | PRO | GLU | GLY | ||||
| 12 | GLY | GLU | LYS | VAL | ILE | VAL | GLY | TYR |
Samples:
sample_1: ERH-2, [U-100% 13C; U-100% 15N; U-80% 2H], 600 ± 50 uM
sample_conditions_1: ionic strength: 0.25 M; pH: 7.42; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.9.1.7 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts