BMRB Entry 50916
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50916
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Title: Backbone 1H, 13C and 15N chemical shift assignments of HIV-1 p6. PubMed: 34342986
Deposition date: 2021-04-28 Original release date: 2021-07-29
Authors: Ramaraju, Bhargavi; Deshmukh, Lalit
Citation: Ramaraju, Bhargavi; Nelson, Spencer; Zheng, Wenwei; Ghirlando, Rodolfo; Deshmukh, Lalit. "Quantitative NMR Study of Insulin-Degrading Enzyme Using Amyloid-beta and HIV-1 p6 Elucidates Its Chaperone Activity" Biochemistry 60, 2519-2523 (2021).
Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available
Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-1
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
entity_1: SRPEPTAPPEESFRSGVETT
TPPQKQEPIDKELYPLTSLR
SLFGNDPSSQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 109 |
| 15N chemical shifts | 36 |
| 1H chemical shifts | 36 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HIV-1 p6 | 1 |
Entities:
Entity 1, HIV-1 p6 50 residues - Formula weight is not available
| 1 | SER | ARG | PRO | GLU | PRO | THR | ALA | PRO | PRO | GLU | |
| 2 | GLU | SER | PHE | ARG | SER | GLY | VAL | GLU | THR | THR | |
| 3 | THR | PRO | PRO | GLN | LYS | GLN | GLU | PRO | ILE | ASP | |
| 4 | LYS | GLU | LEU | TYR | PRO | LEU | THR | SER | LEU | ARG | |
| 5 | SER | LEU | PHE | GLY | ASN | ASP | PRO | SER | SER | GLN |
Samples:
sample_1: HIV-1 p6, [U-100% 13C; U-100% 15N], 0.75 mM; TRIS 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 275.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts