BMRB Entry 50942

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50942

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Title:
NMR backbone resonance assignment of the pro-apoptotic Bak in its apo state
Deposition date:
2021-05-14
Original release date:
2021-06-15
Authors:
Sperl, Laura; Hagn, Franz
Citation:

Citation: Sperl, Laura; Ruehrnoessl, Florian; Schiller, Anita; Haslbeck, Martin; Hagn, Franz. "High-resolution analysis of the conformational transition of pro-apoptotic Bak at the lipid membrane"  EMBO J. 40, e107159-e107159 (2021).
PubMed: 34523144

Assembly members:

Assembly members:
entity_1, polymer, 214 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEASASGQGPGPPRQECGEP ALPSASEEQVAQDTEEVFRS YVFYRHQQEQEAEGVAAPAD PEMVTLPLQPSSTMGQVGRQ LAIIGDDINRRYDSEFQTML QHLQPTAENAYEYFTKIATS LFESGINWGRVVALLGFGYR LALHVYQHGLTGFLGQVTRF VVDFMLHHCIARWIAQRGGW VAALNLGNGPILNVLVVLGV VLLGQFVVRRFFKS

Data sets:
Data typeCount
13C chemical shifts398
15N chemical shifts139
1H chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Bak1

Entities:

Entity 1, Bak 214 residues - Formula weight is not available

1   METGLUALASERALASERGLYGLNGLYPRO
2   GLYPROPROARGGLNGLUCYSGLYGLUPRO
3   ALALEUPROSERALASERGLUGLUGLNVAL
4   ALAGLNASPTHRGLUGLUVALPHEARGSER
5   TYRVALPHETYRARGHISGLNGLNGLUGLN
6   GLUALAGLUGLYVALALAALAPROALAASP
7   PROGLUMETVALTHRLEUPROLEUGLNPRO
8   SERSERTHRMETGLYGLNVALGLYARGGLN
9   LEUALAILEILEGLYASPASPILEASNARG
10   ARGTYRASPSERGLUPHEGLNTHRMETLEU
11   GLNHISLEUGLNPROTHRALAGLUASNALA
12   TYRGLUTYRPHETHRLYSILEALATHRSER
13   LEUPHEGLUSERGLYILEASNTRPGLYARG
14   VALVALALALEULEUGLYPHEGLYTYRARG
15   LEUALALEUHISVALTYRGLNHISGLYLEU
16   THRGLYPHELEUGLYGLNVALTHRARGPHE
17   VALVALASPPHEMETLEUHISHISCYSILE
18   ALAARGTRPILEALAGLNARGGLYGLYTRP
19   VALALAALALEUASNLEUGLYASNGLYPRO
20   ILELEUASNVALLEUVALVALLEUGLYVAL
21   VALLEULEUGLYGLNPHEVALVALARGARG
22   PHEPHELYSSER

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks