BMRB Entry 50956

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50956

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Title:
Side chain 1H, 13C, 15N chemical shift assignments of lysine residues in delta+PHS/V66K staphylococcal nuclease selectively labeled with SAIL Lysine
Deposition date:
2021-05-28
Original release date:
2021-06-10
Authors:
Takeda, Mitsuhiro; Kainosho, Masatsune
Citation:

Citation: Takeda, Mitsuhiro; Miyanoiri, Yohei; Terauchi, Tsutomu; Kainosho, Masatsune. "Conformational features and ionization states of Lys side chains in a protein studied using the stereo-array isotope labeling (SAIL) method"  Magn. Reson. 2, 223-237 (2021).
PubMed: 37904773

Assembly members:

Assembly members:
entity_1, polymer, 164 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MATSTKKLHKEPATLIKAID GDTVKLMYKGQPMTFRLLLV DTPEFNEKYGPEASAFTKKM KENAKKIEVEFDKGQRTDKY GRGLAYIYADGKMVNEALVR QGLAKVAYVYKGNNTHEQLL RKAEAQAKKEKLNIWSEDNA DSGQ

Data sets:
Data typeCount
13C chemical shifts105
15N chemical shifts21
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1D+PHS/V66K1

Entities:

Entity 1, D+PHS/V66K 164 residues - Formula weight is not available

Amino acid mutations (P117G, H124L, S128A, G50F, V51N) were introduced and residue number 44 to 49 (TKHPKK) were deleted.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALATHRSERTHRLYSLYSLEUHISLYS
4   GLUPROALATHRLEUILELYSALAILEASP
5   GLYASPTHRVALLYSLEUMETTYRLYSGLY
6   GLNPROMETTHRPHEARGLEULEULEUVAL
7   ASPTHRPROGLUPHEASNGLULYSTYRGLY
8   PROGLUALASERALAPHETHRLYSLYSMET
9   LYSGLUASNALALYSLYSILEGLUVALGLU
10   PHEASPLYSGLYGLNARGTHRASPLYSTYR
11   GLYARGGLYLEUALATYRILETYRALAASP
12   GLYLYSMETVALASNGLUALALEUVALARG
13   GLNGLYLEUALALYSVALALATYRVALTYR
14   LYSGLYASNASNTHRHISGLUGLNLEULEU
15   ARGLYSALAGLUALAGLNALALYSLYSGLU
16   LYSLEUASNILETRPSERGLUASPASNALA
17   ASPSERGLYGLN