BMRB Entry 5096

Name: Solution Structure of a CCHH mutant of the ninth CCHC Zinc Finger of U-shaped
Published: 2003-06-02

Click here to enlarge.

PDB ID: 1jn7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5096
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution Structure of a CCHH mutant of the ninth CCHC Zinc Finger of U-shaped

Deposition date:

2001-08-02

Original release date:

2003-06-02

Authors:

Kowalski, K.; Mackay, J.

Citation:

Citation: Kowalski, K.; Liew, C.; Matthews, J.; Gell, D.; Crossley, M.; Mackay, J.. "Characterization of the Conserved Interaction between GATA and FOG Family Proteins" J. Biol. Chem. 277, 35720-35729 (2002).
PubMed: 12110675

Assembly members:

Assembly members:
U-shaped ninth zinc finger domain, polymer, 36 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U-shaped ninth zinc finger domain: GSAAEVMKKYCSTCDISFNY VKTYLAHKQFYHKNKP

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
coupling_constants
- J_values_set_1

Data type	Count
1H chemical shifts	208
coupling constants	24

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	U-shaped transcriptional cofactor	1
2	ZINC ION	2

Entities:

Entity 1, U-shaped transcriptional cofactor 36 residues - Formula weight is not available

1

GLY

SER

ALA

GLU

VAL

MET

LYS

TYR

2

CYS

SER

THR

CYS

ASP

ILE

SER

PHE

ASN

TYR

3

VAL

LYS

THR

TYR

LEU

ALA

HIS

LYS

GLN

PHE

4

TYR

HIS

LYS

ASN

LYS

PRO

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: U-shaped ninth zinc finger domain 1.0 mM; ZnSO4 1.5 mM; TCEP 1.5 mM

sample_2: U-shaped ninth zinc finger domain, [U-95% 15N], 0.5 mM; ZnSO4 0.75 mM; TCEP 0.75 mM

sample_cond_1: ionic strength: 1.5 mM; pH: 5.0; pressure: 1 atm; temperature: 293 K

sample_cond_2: ionic strength: 0.75 mM; pH: 5.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
TOCSY	not available	not available	not available
DQF-COSY	not available	not available	not available
E-COSY	not available	not available	not available
HNHA	not available	not available	not available

Software:

xwinnmr v2.6 - processing

DYANA v1.5 - structure solution

CNS v0.5 - refinement

NMR spectrometers:

Bruker DRX 600 MHz