BMRB Entry 50961

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50961

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Title:
1H, 15N and 13C chemical shift assignments of the PYK2(728-839) polypeptide
Deposition date:
2021-06-05
Original release date:
2022-10-12
Authors:
Momin, Afaque Ahmad; Mendes, Tiago; Barthe, Philippe; Faure, Camille; Hong, SeungBeom; Jaremko, Mariusz; Girault, Jean-Antoine; Jaremko, Lukasz; Arold, Stefan
Citation:

Citation: Momin, Afaque; Mendes, Tiago; Barthe, Philippe; Faure, Camille; Hong, SeungBeom; Yu, Piao; Kadare, Gress; Jaremko, Mariusz; Girault, Jean-Antoine; Jaremko, Lukasz; Arold, Stefan. "PYK2 senses calcium through a disordered dimerization and calmodulin-binding element"  Commun. Biol. 5, 800-800 (2022).
PubMed: 35945264

Assembly members:

Assembly members:
entity_1, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHHSSGLVPRGSTNLL APKLQFQVPEGLCASSPTLT SPMEYPSPVNSLHTPPLHRH NVFKRHSMREEDFIQPSSRE EAQQLWEAEKVKMRQILDKQ QKQMVEDYQWLRQEEKSLDP MVYMNDKS

Data sets:
Data typeCount
13C chemical shifts331
15N chemical shifts94
1H chemical shifts708

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PYK2(728-839) polypeptide1

Entities:

Entity 1, PYK2(728-839) polypeptide 128 residues - Formula weight is not available

1   METHISHISHISHISHISHISSERSERGLY
2   LEUVALPROARGGLYSERTHRASNLEULEU
3   ALAPROLYSLEUGLNPHEGLNVALPROGLU
4   GLYLEUCYSALASERSERPROTHRLEUTHR
5   SERPROMETGLUTYRPROSERPROVALASN
6   SERLEUHISTHRPROPROLEUHISARGHIS
7   ASNVALPHELYSARGHISSERMETARGGLU
8   GLUASPPHEILEGLNPROSERSERARGGLU
9   GLUALAGLNGLNLEUTRPGLUALAGLULYS
10   VALLYSMETARGGLNILELEUASPLYSGLN
11   GLNLYSGLNMETVALGLUASPTYRGLNTRP
12   LEUARGGLNGLUGLULYSSERLEUASPPRO
13   METVALTYRMETASNASPLYSSER

Related Database Links:

UniProt Q14289
AlphaFold Q6PID4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks