BMRB Entry 50962
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50962
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Title: Backbone 1H, 13C and 15N chemical shift assignment of C-terminal polybasic region of human Semaphorin 3A PubMed: 34577606
Deposition date: 2021-06-07 Original release date: 2021-08-04
Authors: Perez, Yolanda; Bonet, Roman; Corredor, Miriam; Domingo, Cecilia; Moure, Alejandra; Bujons, Jordi; Messeguer, Angel; Alfonso, Ignacio
Citation: Perez, Yolanda; Bonet, Roman; Corredor, Miriam; Domingo, Cecilia; Moure, Alejandra; Messeguer, Angel; Bujons, Jordi; Alfonso, Ignacio. "Semaphorin 3A - Glycosaminoglycans interaction as therapeu-tic target for axonal regeneration" Pharmaceuticals (Basel) 14, 906-906 (2021).
Assembly members:
entity_1, polymer, 48 residues, 5915 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET45
Entity Sequences (FASTA):
entity_1: AVWKRDRKQRRQRPGHTPGN
SNKWKHLQENKKGRNRRTHE
FERAPRSV
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 140 |
15N chemical shifts | 47 |
1H chemical shifts | 284 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | C-terminal polybasic domain Sema3A | 1 |
Entities:
Entity 1, C-terminal polybasic domain Sema3A 48 residues - 5915 Da.
1 | ALA | VAL | TRP | LYS | ARG | ASP | ARG | LYS | GLN | ARG | ||||
2 | ARG | GLN | ARG | PRO | GLY | HIS | THR | PRO | GLY | ASN | ||||
3 | SER | ASN | LYS | TRP | LYS | HIS | LEU | GLN | GLU | ASN | ||||
4 | LYS | LYS | GLY | ARG | ASN | ARG | ARG | THR | HIS | GLU | ||||
5 | PHE | GLU | ARG | ALA | PRO | ARG | SER | VAL |
Samples:
sample_1: C-terminal polybasic domain of Semaphorin 3A, [U-100% 13C; U-100% 15N], 1 mM; acetate 10 mM; NaCl 50 mM
sample_conditions_1: ionic strength: 60 mM; pH: 5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5.7 - collection, data analysis
CcpNMR v2.4 - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Bruker 500 MHz Avance HDIII 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts