BMRB Entry 50964
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50964
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Title: yRad6 PubMed: 36162503
Deposition date: 2021-06-07 Original release date: 2023-03-01
Authors: Shukla, Prakash; Skalicky, Jack; Chandrasekharan, Mahesh
Citation: Shukla, Prakash; Sinha, Dhiraj; Leng, Andrew; Bissell, Jesse; Thatipamula, Shravya; Ganguly, Rajarshi; Radmall, Kaitlin; Skalicky, Jack; Shrieve, Dennis; Chandrasekharan, Mahesh. "Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 in helix-3 affect ubiquitination activity and decrease enzyme stability" J. Biol. Chem. 298, 102524-102524 (2022).
Assembly members:
entity_1, polymer, 150 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae Vector: unknown
Entity Sequences (FASTA):
entity_1: MSTPARRRLMRDFKRMKEDA
PPGVSASPLPDNVMVWNAMI
IGPADTPYEDGTFRLLLEFD
EEYPNKPPHVKFLSEMFHPN
VYANGEICLDILQNRWTPTY
DVASILTSIQSLFNDPNPAS
PANVEAATLFKDHKSQYVKR
VKETVEKSWE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 291 |
| 15N chemical shifts | 129 |
| 1H chemical shifts | 129 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | yRad6 | 1 |
Entities:
Entity 1, yRad6 150 residues - Formula weight is not available
| 1 | MET | SER | THR | PRO | ALA | ARG | ARG | ARG | LEU | MET | |
| 2 | ARG | ASP | PHE | LYS | ARG | MET | LYS | GLU | ASP | ALA | |
| 3 | PRO | PRO | GLY | VAL | SER | ALA | SER | PRO | LEU | PRO | |
| 4 | ASP | ASN | VAL | MET | VAL | TRP | ASN | ALA | MET | ILE | |
| 5 | ILE | GLY | PRO | ALA | ASP | THR | PRO | TYR | GLU | ASP | |
| 6 | GLY | THR | PHE | ARG | LEU | LEU | LEU | GLU | PHE | ASP | |
| 7 | GLU | GLU | TYR | PRO | ASN | LYS | PRO | PRO | HIS | VAL | |
| 8 | LYS | PHE | LEU | SER | GLU | MET | PHE | HIS | PRO | ASN | |
| 9 | VAL | TYR | ALA | ASN | GLY | GLU | ILE | CYS | LEU | ASP | |
| 10 | ILE | LEU | GLN | ASN | ARG | TRP | THR | PRO | THR | TYR | |
| 11 | ASP | VAL | ALA | SER | ILE | LEU | THR | SER | ILE | GLN | |
| 12 | SER | LEU | PHE | ASN | ASP | PRO | ASN | PRO | ALA | SER | |
| 13 | PRO | ALA | ASN | VAL | GLU | ALA | ALA | THR | LEU | PHE | |
| 14 | LYS | ASP | HIS | LYS | SER | GLN | TYR | VAL | LYS | ARG | |
| 15 | VAL | LYS | GLU | THR | VAL | GLU | LYS | SER | TRP | GLU |
Samples:
sample_1: potassium phosphate 25 mM; TCEP 0.5 mM; Rad6, [U-100% 13C; U-100% 15N], 0.75 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts