BMRB Entry 50966
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50966
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Title: 1H, 15N, 13C resonnance assigments and secondary structure of PulL, a component of the Klebsiella oxytoca type II secretion system PubMed: 34410621
Deposition date: 2021-06-09 Original release date: 2021-08-27
Authors: Dazzoni, Regine; Lopez-Castilla, Aracelys; Cordier, Florence; Bardiaux, Benjamin; Nilges, Michael; Izadi-Pruneyre, Nadia
Citation: Dazzoni, Regine; Lopez-Castilla, Aracelys; Cordier, Florence; Bardiaux, Benjamin; Nilges, Michael; Francetic, Olivera; Izadi-Pruneyre, Nadia. "1H, 15N and 13C resonance assignments of the C-terminal domain of PulL, a component of the Klebsiella oxytoca type II secretion system" Biomol. NMR Assignments 15, 455-459 (2021).
Assembly members:
entity_1, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: Klebsiella oxytoca Taxonomy ID: 571 Superkingdom: Bacteria Kingdom: not available Genus/species: Klebsiella oxytoca
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMalp2
Entity Sequences (FASTA):
entity_1: SNAGARPALISRLGALQQII
DDTPGIRLRTLSFDAARNAL
QLEISAVSSQALEQFSQRAR
ARFRVQTGEMKPRADGIEGR
LTLEGNDA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 317 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 471 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PulL CTD | 1 |
Entities:
Entity 1, PulL CTD 88 residues - Formula weight is not available
| 1 | SER | ASN | ALA | GLY | ALA | ARG | PRO | ALA | LEU | ILE | ||||
| 2 | SER | ARG | LEU | GLY | ALA | LEU | GLN | GLN | ILE | ILE | ||||
| 3 | ASP | ASP | THR | PRO | GLY | ILE | ARG | LEU | ARG | THR | ||||
| 4 | LEU | SER | PHE | ASP | ALA | ALA | ARG | ASN | ALA | LEU | ||||
| 5 | GLN | LEU | GLU | ILE | SER | ALA | VAL | SER | SER | GLN | ||||
| 6 | ALA | LEU | GLU | GLN | PHE | SER | GLN | ARG | ALA | ARG | ||||
| 7 | ALA | ARG | PHE | ARG | VAL | GLN | THR | GLY | GLU | MET | ||||
| 8 | LYS | PRO | ARG | ALA | ASP | GLY | ILE | GLU | GLY | ARG | ||||
| 9 | LEU | THR | LEU | GLU | GLY | ASN | ASP | ALA |
Samples:
sample_1: PulL CTD, [U-100% 13C; U-100% 15N], 450 uM; Hepes 50 mM; NaCl 50 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
ANALYSIS - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts