BMRB Entry 50970

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50970

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Title:
MERS-CoV macro domain free state
Deposition date:
2021-06-11
Original release date:
2021-11-03
Authors:
Gallo, Angelo; Charalampous, Periklis; Tsika, Aikaterini; Fourkiotis, Nikolaos; Spyroulias, Georgios
Citation:

Citation: Tsika, Aikaterini; Fourkiotis, Nikolaos; Charalampous, Periklis; Gallo, Angelo; Spyroulias, Georgios. "NMR study of macro domains (MDs) from betacoronavirus: backbone resonance assignments of SARS-CoV and MERS-CoV MDs in the free and the ADPr-bound state"  Biomol. NMR Assignments 16, 9-16 (2022).
PubMed: 34686999

Assembly members:

Assembly members:
entity_1, polymer, 183 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: MERS-CoV   Taxonomy ID: 1335626   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus MERS-CoV

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: E. cloni(r) EXPRESS BL21(DE3) (Lucigen)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MNVDPLSNFEHKVITECVTI VLGDAIQVAKCYGESVLVNA ANTHLKHGGGIAGAINAASK GAVQKESDEYILAKGPLQVG DSVLLQGHSLAKNILHVVGP DARAKQDVSLLSKCYKAMNA YPLVVTPLVSAGIFGVKPAV SFDYLIREAKTRVLVVVNSQ DVYKSLTIVDIPQLELEHHH HHH

Data sets:
Data typeCount
13C chemical shifts511
15N chemical shifts169
1H chemical shifts169

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MERS-CoV1

Entities:

Entity 1, MERS-CoV 183 residues - Formula weight is not available

The native sequence start with N2 and the native sequence start with N254. We have His-tag the sequence LELEHHHHHH does not belong to the native sequence.

1   METASNVALASPPROLEUSERASNPHEGLU
2   HISLYSVALILETHRGLUCYSVALTHRILE
3   VALLEUGLYASPALAILEGLNVALALALYS
4   CYSTYRGLYGLUSERVALLEUVALASNALA
5   ALAASNTHRHISLEULYSHISGLYGLYGLY
6   ILEALAGLYALAILEASNALAALASERLYS
7   GLYALAVALGLNLYSGLUSERASPGLUTYR
8   ILELEUALALYSGLYPROLEUGLNVALGLY
9   ASPSERVALLEULEUGLNGLYHISSERLEU
10   ALALYSASNILELEUHISVALVALGLYPRO
11   ASPALAARGALALYSGLNASPVALSERLEU
12   LEUSERLYSCYSTYRLYSALAMETASNALA
13   TYRPROLEUVALVALTHRPROLEUVALSER
14   ALAGLYILEPHEGLYVALLYSPROALAVAL
15   SERPHEASPTYRLEUILEARGGLUALALYS
16   THRARGVALLEUVALVALVALASNSERGLN
17   ASPVALTYRLYSSERLEUTHRILEVALASP
18   ILEPROGLNLEUGLULEUGLUHISHISHIS
19   HISHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks