BMRB Entry 50977
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50977
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Title: Human obscurin Ig12 PubMed: 36306263
Deposition date: 2021-06-16 Original release date: 2022-10-24
Authors: Mauriello, Gianna; Wright, Nathan
Citation: Mauriello, Gianna; Moncure, Grace; Nowzari, Roujon; Miller, Callie; Wright, Nathan. "The N-terminus of obscurin is flexible in solution" Proteins 91, 485-496 (2023).
Assembly members:
entity_1, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a
Entity Sequences (FASTA):
entity_1: MKMMFAKEQSVHNEVQAEAG
ASAMLSCEVAQAQTEVTWYK
DGKKLSSSSKVGMEVKGCTR
RLVLPQAGKADAGEYSCEAG
GQRVSFHLHITEPLEHHHHH
H
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 272 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 561 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Human obscurin Ig12 | 1 |
Entities:
Entity 1, Human obscurin Ig12 101 residues - Formula weight is not available
| 1 | MET | LYS | MET | MET | PHE | ALA | LYS | GLU | GLN | SER | ||||
| 2 | VAL | HIS | ASN | GLU | VAL | GLN | ALA | GLU | ALA | GLY | ||||
| 3 | ALA | SER | ALA | MET | LEU | SER | CYS | GLU | VAL | ALA | ||||
| 4 | GLN | ALA | GLN | THR | GLU | VAL | THR | TRP | TYR | LYS | ||||
| 5 | ASP | GLY | LYS | LYS | LEU | SER | SER | SER | SER | LYS | ||||
| 6 | VAL | GLY | MET | GLU | VAL | LYS | GLY | CYS | THR | ARG | ||||
| 7 | ARG | LEU | VAL | LEU | PRO | GLN | ALA | GLY | LYS | ALA | ||||
| 8 | ASP | ALA | GLY | GLU | TYR | SER | CYS | GLU | ALA | GLY | ||||
| 9 | GLY | GLN | ARG | VAL | SER | PHE | HIS | LEU | HIS | ILE | ||||
| 10 | THR | GLU | PRO | LEU | GLU | HIS | HIS | HIS | HIS | HIS | ||||
| 11 | HIS |
Samples:
sample_1: Human obscurin Ig12, [U-100% 15N], 1 mM; TRIS 50 mM; NaCl 20 mM; NaN3 0.35 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 1H-15N heteronoe | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
SPARKY - chemical shift assignment
X-PLOR NIH - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts