BMRB Entry 50997
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50997
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Title: Zinc finger PubMed: 36008487
Deposition date: 2021-06-30 Original release date: 2022-06-27
Authors: Overbeck, Jan; Sprangers, Remco
Citation: Overbeck, Jan; Stelzig, David; Fuchs, Anna-Lisa; Wurm, Jan Philip; Sprangers, Remco. "Observation of conformational changes that underlie the catalytic cycle of Xrn2" Nat. Chem. Biol. 18, 1152-1160 (2022).
Assembly members:
entity_1, polymer, 31 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Chaetomium thermophilum Taxonomy ID: 209285 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Chaetomium thermophilum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETGB1a
Entity Sequences (FASTA):
entity_1: GGEAKARLCKLCGQKGHDER
SCKGEAKQKQG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 117 |
| 15N chemical shifts | 30 |
| 1H chemical shifts | 176 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zinc finger polypeptide | 1 |
| 2 | Zinc ion | 2 |
Entities:
Entity 1, zinc finger polypeptide 31 residues - Formula weight is not available
| 1 | GLY | GLY | GLU | ALA | LYS | ALA | ARG | LEU | CYS | LYS | ||||
| 2 | LEU | CYS | GLY | GLN | LYS | GLY | HIS | ASP | GLU | ARG | ||||
| 3 | SER | CYS | LYS | GLY | GLU | ALA | LYS | GLN | LYS | GLN | ||||
| 4 | GLY |
Entity 2, Zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Zinc finger, [U-13C; U-15N], 0.75 mM; Zn2+ 0.75 mM; HEPES 25 mM; NaCl 125 mM; NaN3 0.03%
sample_conditions_1: ionic strength: 0.125 M; pH: 7.3; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts