BMRB Entry 50998

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50998

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Title:
Backbone assignment of human prolactin at pH 7.0 and 5.5
Deposition date:
2021-06-30
Original release date:
2022-03-03
Authors:
Vang, Janus; Pustovalova, Yulia; Hoch, Jeffrey
Citation:

Citation: Vang, Janus; Pustovalova, Yulia; Korzhnev, Dmitry; Gorbatyuk, Oksana; Keeler, Camille; Hodsdon, Michael; Hoch, Jeffrey. "Architecture of the two metal binding sites in prolactin"  Biophys. J. 121, 1312-1321 (2022).
PubMed: 35192840

Assembly members:

Assembly members:
entity_1, polymer, 199 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LPICPGGAARCQVTLRDLFD RAVVLSHYIHNLSSEMFSEF DKRYTHGRGFITKAINSCHT SSLATPEDKEQAQQMNQKDF LSLIVSILRSWNEPLYHLVT EVRGMQEAPEAILSKAVEIE EQTKRLLEGMELIVSQVHPE TKENEIYPVWSGLPSLQMAD EESRLSAYYNLLHCLRRDSH KIDNYLKLLKCRIIHNNNC

Data sets:
Data typeCount
13C chemical shifts548
15N chemical shifts301
1H chemical shifts301

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1human prolactin1

Entities:

Entity 1, human prolactin 199 residues - Formula weight is not available

1   LEUPROILECYSPROGLYGLYALAALAARG
2   CYSGLNVALTHRLEUARGASPLEUPHEASP
3   ARGALAVALVALLEUSERHISTYRILEHIS
4   ASNLEUSERSERGLUMETPHESERGLUPHE
5   ASPLYSARGTYRTHRHISGLYARGGLYPHE
6   ILETHRLYSALAILEASNSERCYSHISTHR
7   SERSERLEUALATHRPROGLUASPLYSGLU
8   GLNALAGLNGLNMETASNGLNLYSASPPHE
9   LEUSERLEUILEVALSERILELEUARGSER
10   TRPASNGLUPROLEUTYRHISLEUVALTHR
11   GLUVALARGGLYMETGLNGLUALAPROGLU
12   ALAILELEUSERLYSALAVALGLUILEGLU
13   GLUGLNTHRLYSARGLEULEUGLUGLYMET
14   GLULEUILEVALSERGLNVALHISPROGLU
15   THRLYSGLUASNGLUILETYRPROVALTRP
16   SERGLYLEUPROSERLEUGLNMETALAASP
17   GLUGLUSERARGLEUSERALATYRTYRASN
18   LEULEUHISCYSLEUARGARGASPSERHIS
19   LYSILEASPASNTYRLEULYSLEULEULYS
20   CYSARGILEILEHISASNASNASNCYS

Related Database Links:

UNP P01236
AlphaFold Q92996

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks