BMRB Entry 51006
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51006
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Title: Assignment of the full length A6 mouse prion protein at 37degC pH 4 PubMed: 34558139
Deposition date: 2021-07-06 Original release date: 2021-11-10
Authors: Bhate, Suhas; Udgaonkar, Jayant; Das, Ranabir
Citation: Bhate, Suhas; Udgaonkar, Jayant. "Destabilization of polar interactions in the prion protein triggers misfolding and oligomerization" Protein Sci. 30, 2258-2271 (2021).
Assembly members:
entity_1, polymer, 211 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-17b
Entity Sequences (FASTA):
entity_1: MKKRPKPGGWNTGGSRYPGQ
GSPGGNRYPPQGGTWGQPHG
GGWGQPHGGSWGQPHGGSWG
QPHGGGWGQGGGTHNQWNKP
SKPKTNLKHVAGAAAAGAVV
GGLGGYMLGSAMSRPMIHFG
NDWEDRYYRENMYRYPNQVY
YRPVDQYSNQNNFVHDCVNI
TIKQHAAAAAAKGENFTETD
VKMMERVVEQMCVTQYQKES
QAYYDGRRSSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 265 |
| 15N chemical shifts | 158 |
| 1H chemical shifts | 158 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | A6 mouse prion protein | 1 |
Entities:
Entity 1, A6 mouse prion protein 211 residues - Formula weight is not available
| 1 | MET | LYS | LYS | ARG | PRO | LYS | PRO | GLY | GLY | TRP | ||||
| 2 | ASN | THR | GLY | GLY | SER | ARG | TYR | PRO | GLY | GLN | ||||
| 3 | GLY | SER | PRO | GLY | GLY | ASN | ARG | TYR | PRO | PRO | ||||
| 4 | GLN | GLY | GLY | THR | TRP | GLY | GLN | PRO | HIS | GLY | ||||
| 5 | GLY | GLY | TRP | GLY | GLN | PRO | HIS | GLY | GLY | SER | ||||
| 6 | TRP | GLY | GLN | PRO | HIS | GLY | GLY | SER | TRP | GLY | ||||
| 7 | GLN | PRO | HIS | GLY | GLY | GLY | TRP | GLY | GLN | GLY | ||||
| 8 | GLY | GLY | THR | HIS | ASN | GLN | TRP | ASN | LYS | PRO | ||||
| 9 | SER | LYS | PRO | LYS | THR | ASN | LEU | LYS | HIS | VAL | ||||
| 10 | ALA | GLY | ALA | ALA | ALA | ALA | GLY | ALA | VAL | VAL | ||||
| 11 | GLY | GLY | LEU | GLY | GLY | TYR | MET | LEU | GLY | SER | ||||
| 12 | ALA | MET | SER | ARG | PRO | MET | ILE | HIS | PHE | GLY | ||||
| 13 | ASN | ASP | TRP | GLU | ASP | ARG | TYR | TYR | ARG | GLU | ||||
| 14 | ASN | MET | TYR | ARG | TYR | PRO | ASN | GLN | VAL | TYR | ||||
| 15 | TYR | ARG | PRO | VAL | ASP | GLN | TYR | SER | ASN | GLN | ||||
| 16 | ASN | ASN | PHE | VAL | HIS | ASP | CYS | VAL | ASN | ILE | ||||
| 17 | THR | ILE | LYS | GLN | HIS | ALA | ALA | ALA | ALA | ALA | ||||
| 18 | ALA | LYS | GLY | GLU | ASN | PHE | THR | GLU | THR | ASP | ||||
| 19 | VAL | LYS | MET | MET | GLU | ARG | VAL | VAL | GLU | GLN | ||||
| 20 | MET | CYS | VAL | THR | GLN | TYR | GLN | LYS | GLU | SER | ||||
| 21 | GLN | ALA | TYR | TYR | ASP | GLY | ARG | ARG | SER | SER | ||||
| 22 | SER |
Samples:
sample_1: A6 mouse prion protein, [U-99% 13C; U-99% 15N], 500 uM; Sodium Acetate 10 mM
sample_conditions_1: ionic strength: 0.01 M; pH: 4; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts