BMRB Entry 51007

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51007

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Title:
Assignment of the full length Q216R mouse prion protein
Deposition date:
2021-07-06
Original release date:
2021-11-10
Authors:
Bhate, Suhas; Udgaonkar, Jayant; Das, Ranabir
Citation:

Citation: Bhate, Suhas; Udgaonkar, Jayant; Das, Ranabir. "Destabilization of polar interactions in the prion protein triggers misfolding and oligomerization"  Protein Sci. 30, 2258-2271 (2021).
PubMed: 34558139

Assembly members:

Assembly members:
entity_1, polymer, 211 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKKRPKPGGWNTGGSRYPGQ GSPGGNRYPPQGGTWGQPHG GGWGQPHGGSWGQPHGGSWG QPHGGGWGQGGGTHNQWNKP SKPKTNLKHVAGAAAAGAVV GGLGGYMLGSAMSRPMIHFG NDWEDRYYRENMYRYPNQVY YRPVDQYSNQNNFVHDCVNI TIKQHTVTTTTKGENFTETD VKMMERVVEQMCVTRYQKES QAYYDGRRSSS

Data sets:
Data typeCount
13C chemical shifts267
15N chemical shifts155
1H chemical shifts155

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Q216R mo PrP1

Entities:

Entity 1, Q216R mo PrP 211 residues - Formula weight is not available

1   METLYSLYSARGPROLYSPROGLYGLYTRP
2   ASNTHRGLYGLYSERARGTYRPROGLYGLN
3   GLYSERPROGLYGLYASNARGTYRPROPRO
4   GLNGLYGLYTHRTRPGLYGLNPROHISGLY
5   GLYGLYTRPGLYGLNPROHISGLYGLYSER
6   TRPGLYGLNPROHISGLYGLYSERTRPGLY
7   GLNPROHISGLYGLYGLYTRPGLYGLNGLY
8   GLYGLYTHRHISASNGLNTRPASNLYSPRO
9   SERLYSPROLYSTHRASNLEULYSHISVAL
10   ALAGLYALAALAALAALAGLYALAVALVAL
11   GLYGLYLEUGLYGLYTYRMETLEUGLYSER
12   ALAMETSERARGPROMETILEHISPHEGLY
13   ASNASPTRPGLUASPARGTYRTYRARGGLU
14   ASNMETTYRARGTYRPROASNGLNVALTYR
15   TYRARGPROVALASPGLNTYRSERASNGLN
16   ASNASNPHEVALHISASPCYSVALASNILE
17   THRILELYSGLNHISTHRVALTHRTHRTHR
18   THRLYSGLYGLUASNPHETHRGLUTHRASP
19   VALLYSMETMETGLUARGVALVALGLUGLN
20   METCYSVALTHRARGTYRGLNLYSGLUSER
21   GLNALATYRTYRASPGLYARGARGSERSER
22   SER

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SPARKY: Backbone or all simulated peaks