BMRB Entry 51011
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51011
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Title: 1H, 15N, 13C resonance assignment of the N-terminal domain (1-285) of yeast Atg9 PubMed: 35007534
Deposition date: 2021-07-08 Original release date: 2022-01-24
Authors: Coudevylle, Nicolas; Banas, Bartlomiej; Baumann, Verena; Schuschnig, Martina; Zawadzka - Kazimierczuk, Anna; Kozminski, Witkor; Martens, Sascha
Citation: Coudevylle, Nicolas; Banas, Bartlomiej; Baumann, Verena; Schuschnig, Martina; Zawadzka - Kazimierczuk, Anna; Kozminski, Wiktor; Martens, Sascha. "Mechanism of Atg9 recruitment by Atg11 in the cytoplasm-to-vacuole targeting pathway" J. Biol. Chem. 298, 101573-101573 (2022).
Assembly members:
entity_1, polymer, 293 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-M11
Entity Sequences (FASTA):
entity_1: MERDEYQLPNSHGKNTFLSR
IFGLQSDEVNPSLNSQEMSN
FPLPDIERGSSLLHSTNDSR
EDVDENDLRVPESDQGTSTE
EEDEVDEEQVQAYAPQISDG
LDGDHQLNSVTSKENVLETE
KSNLERLVEGSTDDSVPKVG
QLSSEEEEDNEFINNDGFDD
DTPLFQKSKIHEFSSKKSNT
IEDGKRPLFFRHILQNNRPQ
RDTQKLFTSSNAIHHDKDKS
ANNGPRNINGNQKHGTKYFG
SATQPRFTGSPLNNTNRFTK
LFPLRKPNLLSNISVLNNTP
EDRINHMLEVLFQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 612 |
| 15N chemical shifts | 216 |
| 1H chemical shifts | 784 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Atg9-NTD | 1 |
Entities:
Entity 1, Atg9-NTD 293 residues - Formula weight is not available
| 1 | MET | GLU | ARG | ASP | GLU | TYR | GLN | LEU | PRO | ASN | ||||
| 2 | SER | HIS | GLY | LYS | ASN | THR | PHE | LEU | SER | ARG | ||||
| 3 | ILE | PHE | GLY | LEU | GLN | SER | ASP | GLU | VAL | ASN | ||||
| 4 | PRO | SER | LEU | ASN | SER | GLN | GLU | MET | SER | ASN | ||||
| 5 | PHE | PRO | LEU | PRO | ASP | ILE | GLU | ARG | GLY | SER | ||||
| 6 | SER | LEU | LEU | HIS | SER | THR | ASN | ASP | SER | ARG | ||||
| 7 | GLU | ASP | VAL | ASP | GLU | ASN | ASP | LEU | ARG | VAL | ||||
| 8 | PRO | GLU | SER | ASP | GLN | GLY | THR | SER | THR | GLU | ||||
| 9 | GLU | GLU | ASP | GLU | VAL | ASP | GLU | GLU | GLN | VAL | ||||
| 10 | GLN | ALA | TYR | ALA | PRO | GLN | ILE | SER | ASP | GLY | ||||
| 11 | LEU | ASP | GLY | ASP | HIS | GLN | LEU | ASN | SER | VAL | ||||
| 12 | THR | SER | LYS | GLU | ASN | VAL | LEU | GLU | THR | GLU | ||||
| 13 | LYS | SER | ASN | LEU | GLU | ARG | LEU | VAL | GLU | GLY | ||||
| 14 | SER | THR | ASP | ASP | SER | VAL | PRO | LYS | VAL | GLY | ||||
| 15 | GLN | LEU | SER | SER | GLU | GLU | GLU | GLU | ASP | ASN | ||||
| 16 | GLU | PHE | ILE | ASN | ASN | ASP | GLY | PHE | ASP | ASP | ||||
| 17 | ASP | THR | PRO | LEU | PHE | GLN | LYS | SER | LYS | ILE | ||||
| 18 | HIS | GLU | PHE | SER | SER | LYS | LYS | SER | ASN | THR | ||||
| 19 | ILE | GLU | ASP | GLY | LYS | ARG | PRO | LEU | PHE | PHE | ||||
| 20 | ARG | HIS | ILE | LEU | GLN | ASN | ASN | ARG | PRO | GLN | ||||
| 21 | ARG | ASP | THR | GLN | LYS | LEU | PHE | THR | SER | SER | ||||
| 22 | ASN | ALA | ILE | HIS | HIS | ASP | LYS | ASP | LYS | SER | ||||
| 23 | ALA | ASN | ASN | GLY | PRO | ARG | ASN | ILE | ASN | GLY | ||||
| 24 | ASN | GLN | LYS | HIS | GLY | THR | LYS | TYR | PHE | GLY | ||||
| 25 | SER | ALA | THR | GLN | PRO | ARG | PHE | THR | GLY | SER | ||||
| 26 | PRO | LEU | ASN | ASN | THR | ASN | ARG | PHE | THR | LYS | ||||
| 27 | LEU | PHE | PRO | LEU | ARG | LYS | PRO | ASN | LEU | LEU | ||||
| 28 | SER | ASN | ILE | SER | VAL | LEU | ASN | ASN | THR | PRO | ||||
| 29 | GLU | ASP | ARG | ILE | ASN | HIS | MET | LEU | GLU | VAL | ||||
| 30 | LEU | PHE | GLN |
Samples:
sample_1: Atg9-NTD, [U-95% 13C; U-95% 15N], 0,4 mM; Bis-Tris 25 mM; sodium chloride 300 mM
sample_conditions_1: ionic strength: 325 mM; pH: 6; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 5D HN(CA)CONH | sample_1 | isotropic | sample_conditions_1 |
| 5D (HACA)CON(CA)CONH | sample_1 | isotropic | sample_conditions_1 |
| 5D HabCabCONH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - experiments setup and acquisition
SPARKY - spectra display, peak picking
TSAR - resonance assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts