warning: failed to read freetag - ' _nef_spectrum_dimension\.is_acquisition'
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warning: failed to read freetag - ' _nef_spectrum_dimension\.is_acquisition'
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warning: failed to read freetag - ' _nef_spectrum_dimension\.is_acquisition'
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BMRB Entry 51027 BMRB - Biological Magnetic Resonance Bank

BMRB Entry 51027

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51027

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Title:
E14Q-EmrE drug free, low pH
Deposition date:
2021-07-23
Original release date:
2022-06-08
Authors:
Tonelli, Marco; Thomas, Nathan; Spreacker, Peyton; Henzler-Wildman, Katherine
Citation:

Citation: Spreacker, Peyton; Thomas, Nathan; Beeninga, Will; Brousseau, Merissa; Porter, Colin; Hibbs, Kylie; Henzler-Wildman, Katherine. "Activating alternative transport modes in a multidrug resistance efflux pump to confer chemical susceptibility"  Nat. Commun. 13, 7655-7655 (2022).
PubMed: 36496486

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, 11910 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MNPYIYLGGAILAQVIGTTL MKFSEGFTRLWPSVGTIICY CASFWLLAQTLAYIPTGIAY AIWSGVGIVLISLLSWGFFG QRLDLPAIIGMMLICAGVLI INLLSRSTPH

Data typeCount
13C chemical shifts291
15N chemical shifts153
1H chemical shifts153

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1E14Q-EmrE, Chain A1
2E14Q-EmrE, Chain B1

Entities:

Entity 1, E14Q-EmrE, Chain A 110 residues - 11910 Da.

1   METASNPROTYRILETYRLEUGLYGLYALA
2   ILELEUALAGLNVALILEGLYTHRTHRLEU
3   METLYSPHESERGLUGLYPHETHRARGLEU
4   TRPPROSERVALGLYTHRILEILECYSTYR
5   CYSALASERPHETRPLEULEUALAGLNTHR
6   LEUALATYRILEPROTHRGLYILEALATYR
7   ALAILETRPSERGLYVALGLYILEVALLEU
8   ILESERLEULEUSERTRPGLYPHEPHEGLY
9   GLNARGLEUASPLEUPROALAILEILEGLY
10   METMETLEUILECYSALAGLYVALLEUILE
11   ILEASNLEULEUSERARGSERTHRPROHIS

Download HSQC peak lists in one of the following formats:
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