BMRB Entry 51039
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51039
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Title: 1H, 13C, 15N Chemical Shift Assignments for ERD14 K-segment B PubMed: 34963932
Deposition date: 2021-07-29 Original release date: 2022-02-04
Authors: Sebak, Fanni; Bodor, Andrea
Citation: Sebak, Fanni; Horvath, Lilla; Kovacs, Daniel; Szolomajer, Janos; Toth, Gabor; Babiczky, Akos; Bosze, Szilvia; Bodor, Andrea. "Novel Lysine-Rich Delivery Peptides of Plant Origin ERD and Human S100: The Effect of Carboxyfluorescein Conjugation, Influence of Aromatic and Proline Residues, Cellular Internalization, and Penetration Ability" ACS Omega 6, 34470-34484 (2021).
Assembly members:
entity_1, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: EKKGFMEKLKEKLPG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 15 |
15N chemical shifts | 13 |
1H chemical shifts | 30 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ERDB | 1 |
Entities:
Entity 1, ERDB 15 residues - Formula weight is not available
1 | GLU | LYS | LYS | GLY | PHE | MET | GLU | LYS | LEU | LYS | ||||
2 | GLU | LYS | LEU | PRO | GLY |
Samples:
sample_1: ERDB 1 mM; H2O 450 uL; D2O, [U-100% 2H], 50 uL; DSS 5 uL
sample_conditions_1: pH: 3; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.6.0 - processing
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts