BMRB Entry 5105
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5105
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Title: An NMR Approach to Structural Proteomics PubMed: 11854485
Deposition date: 2001-08-14 Original release date: 2002-04-08
Authors: Yee, Adelinda; Chang, Xiaoqing; Pineda-Lucena, Antonio; Wu, Bin; Semesi, Anthony; Le, Brian; Ramelot, Theresa; Lee, Gregory; Bhattacharyya, Sudeepa; Gutierrez, Pablo; Denisov, Aleksej; Lee, Chang-Hun; Cort, John; Kozlov, Guennadi; Liao, Jack; Finak, Grzegorz; Chen, Limin; Wishart, David; Lee, Weontae; McIntosh, Lawrence; Gehring, Kalle; Kennedy, Michael; Edwards, Aled; Arrowsmith, Cheryl
Citation: Yee, Adelinda; Chang, Xiaoqing; Pineda-Lucena, Antonio; Wu, Bin; Semesi, Anthony; Le, Brian; Ramelot, Theresa; Lee, Gregory; Bhattacharyya, Sudeepa; Gutierrez, Pablo; Denisov, Aleksej; Lee, Chang-Hun; Cort, John; Kozlov, Guennadi; Liao, Jack; Finak, Grzegorz; Chen, Limin; Wishart, David; Lee, Weontae; McIntosh, Lawrence; Gehring, Kalle; Kennedy, Michael; Edwards, Aled; Arrowsmith, Cheryl. "An NMR Approach to Structural Proteomics" Proc. Natl. Acad. Sci. U.S.A. 99, 1825-1830 (2002).
Assembly members:
EC298, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
EC298: MNKDEAGGNWKQFKGKVKEQ
WGKLTDDDMTIIEGKRDQLV
GKIQERYGYQKDQAEKEVVD
WETRNEYRW
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 433 |
| 13C chemical shifts | 209 |
| 15N chemical shifts | 66 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EC298 | 1 |
Entities:
Entity 1, EC298 69 residues - Formula weight is not available
| 1 | MET | ASN | LYS | ASP | GLU | ALA | GLY | GLY | ASN | TRP | ||||
| 2 | LYS | GLN | PHE | LYS | GLY | LYS | VAL | LYS | GLU | GLN | ||||
| 3 | TRP | GLY | LYS | LEU | THR | ASP | ASP | ASP | MET | THR | ||||
| 4 | ILE | ILE | GLU | GLY | LYS | ARG | ASP | GLN | LEU | VAL | ||||
| 5 | GLY | LYS | ILE | GLN | GLU | ARG | TYR | GLY | TYR | GLN | ||||
| 6 | LYS | ASP | GLN | ALA | GLU | LYS | GLU | VAL | VAL | ASP | ||||
| 7 | TRP | GLU | THR | ARG | ASN | GLU | TYR | ARG | TRP |
Samples:
SAMPLE_1: EC298, [U-13C; U-15N], 1.0 mM
EX-COND_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- VARIAN INOVA 500 MHz
- VARIAN INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB38451 BAE78047 BAG79861 BAI28306 BAI33483 |
| EMBL | CAQ34394 CAR01024 CAR15697 CAV01331 CBG37238 |
| GB | AAC43139 AAC77015 AAG59244 AAN45582 AAP18617 |
| REF | NP_290679 NP_313055 NP_418469 NP_709875 NP_838806 |
| SP | P68206 P68207 P68208 |
| AlphaFold | P68206 P68207 P68208 |
Download HSQC peak lists in one of the following formats:
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