BMRB Entry 51059
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51059
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Title: NMR assignments of the Fc fragment of human immunoglobulin G1 glycoprotein non-fucosylated, non-galactosylated (G0) glycoform PubMed: 34978013
Deposition date: 2021-08-17 Original release date: 2022-03-11
Authors: Yanaka, Saeko; Yagi, Hirokazu; Yogo, Rina; Onitsuka, Masayoshi; Kato, Koichi
Citation: Yanaka, Saeko; Yagi, Hirokazu; Yogo, Rina; Onitsuka, Masayoshi; Kato, Koichi. "Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1" J. Biomol. NMR 76, 17-22 (2022).
Assembly members:
entity_1, polymer, 222 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Chinese hamster ovary cells Vector: Mammalian PowerExpress system vector
Entity Sequences (FASTA):
entity_1: TCPPCPAPELLGGPSVFLFP
PKPKDTLMISRTPEVTCVVV
DVSHEDPEVKFNWYVDGVEV
HNAKTKPREEQYNSTYRVVS
VLTVLHQDWLNGKEYKCKVS
NKALPAPIEKTISKAKGQPR
EPQVYTLPPSRDELTKNQVS
LTCLVKGFYPSDIAVEWESN
GQPENNYKTTPPVLDSDGSF
FLYSKLTVDKSRWQQGNVFS
CSVMHEALHNHYTQKSLSLS
PG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 180 |
| 1H chemical shifts | 180 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1, chain 1 | 1 |
| 2 | entity_1, chain 2 | 1 |
Entities:
Entity 1, entity_1, chain 1 222 residues - Formula weight is not available
| 1 | THR | CYS | PRO | PRO | CYS | PRO | ALA | PRO | GLU | LEU | ||||
| 2 | LEU | GLY | GLY | PRO | SER | VAL | PHE | LEU | PHE | PRO | ||||
| 3 | PRO | LYS | PRO | LYS | ASP | THR | LEU | MET | ILE | SER | ||||
| 4 | ARG | THR | PRO | GLU | VAL | THR | CYS | VAL | VAL | VAL | ||||
| 5 | ASP | VAL | SER | HIS | GLU | ASP | PRO | GLU | VAL | LYS | ||||
| 6 | PHE | ASN | TRP | TYR | VAL | ASP | GLY | VAL | GLU | VAL | ||||
| 7 | HIS | ASN | ALA | LYS | THR | LYS | PRO | ARG | GLU | GLU | ||||
| 8 | GLN | TYR | ASN | SER | THR | TYR | ARG | VAL | VAL | SER | ||||
| 9 | VAL | LEU | THR | VAL | LEU | HIS | GLN | ASP | TRP | LEU | ||||
| 10 | ASN | GLY | LYS | GLU | TYR | LYS | CYS | LYS | VAL | SER | ||||
| 11 | ASN | LYS | ALA | LEU | PRO | ALA | PRO | ILE | GLU | LYS | ||||
| 12 | THR | ILE | SER | LYS | ALA | LYS | GLY | GLN | PRO | ARG | ||||
| 13 | GLU | PRO | GLN | VAL | TYR | THR | LEU | PRO | PRO | SER | ||||
| 14 | ARG | ASP | GLU | LEU | THR | LYS | ASN | GLN | VAL | SER | ||||
| 15 | LEU | THR | CYS | LEU | VAL | LYS | GLY | PHE | TYR | PRO | ||||
| 16 | SER | ASP | ILE | ALA | VAL | GLU | TRP | GLU | SER | ASN | ||||
| 17 | GLY | GLN | PRO | GLU | ASN | ASN | TYR | LYS | THR | THR | ||||
| 18 | PRO | PRO | VAL | LEU | ASP | SER | ASP | GLY | SER | PHE | ||||
| 19 | PHE | LEU | TYR | SER | LYS | LEU | THR | VAL | ASP | LYS | ||||
| 20 | SER | ARG | TRP | GLN | GLN | GLY | ASN | VAL | PHE | SER | ||||
| 21 | CYS | SER | VAL | MET | HIS | GLU | ALA | LEU | HIS | ASN | ||||
| 22 | HIS | TYR | THR | GLN | LYS | SER | LEU | SER | LEU | SER | ||||
| 23 | PRO | GLY |
Samples:
sample_1: Fc fragment of human immunoglobulin G1 glycoprotein non-fucosylated, non-galactosylated (G0) glycoform, [U-100% 15N], 10 mg/mL; NaCl 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 315 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, NMRview - chemical shift assignment, collection
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts