BMRB Entry 51062
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51062
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Title: Backbone assignment of ATF4 transactivation domain PubMed: 35077711
Deposition date: 2021-08-23 Original release date: 2021-09-18
Authors: Siang, Steven; Roche, Julien
Citation: Siang, Steven; Underbakke, Eric; Roche, Julien. "Intricate coupling between the transactivation and basic-leucine zipper domains governs phosphorylation of transcription factor ATF4 by casein kinase 2" J. Biol. Chem. 298, 101633-101633 (2022).
Assembly members:
entity_1, polymer, 275 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T1
Entity Sequences (FASTA):
entity_1: MTEMSFLSSEVLVGDLMSPF
DQSGLGAEESLGLLDDYLEV
AKHFKPHGFSSDKAKAGSSE
WLAVDGLVSPSNNSKEDAFS
GTDWMLEKMDLKEFDLDALL
GIDDLETMPDDLLTTLDDTC
DLFAPLVQETNKQPPQTVNP
IGHLPESLTKPDQVAPFTFL
QPLPLSPGVLSSTPDHSFSL
ELGSEVDITEGDRKPDYTAY
VAMIPQCIKEEDTPSDNDSG
ICMSPESYLGSPQHSPSTRG
SPNRSLPSPGVLCGSARPKP
YDPPGEKMVAAKVKG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 687 |
| 15N chemical shifts | 196 |
| 1H chemical shifts | 196 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ATF4 transactivation domain | 1 |
Entities:
Entity 1, ATF4 transactivation domain 275 residues - Formula weight is not available
| 1 | MET | THR | GLU | MET | SER | PHE | LEU | SER | SER | GLU | ||||
| 2 | VAL | LEU | VAL | GLY | ASP | LEU | MET | SER | PRO | PHE | ||||
| 3 | ASP | GLN | SER | GLY | LEU | GLY | ALA | GLU | GLU | SER | ||||
| 4 | LEU | GLY | LEU | LEU | ASP | ASP | TYR | LEU | GLU | VAL | ||||
| 5 | ALA | LYS | HIS | PHE | LYS | PRO | HIS | GLY | PHE | SER | ||||
| 6 | SER | ASP | LYS | ALA | LYS | ALA | GLY | SER | SER | GLU | ||||
| 7 | TRP | LEU | ALA | VAL | ASP | GLY | LEU | VAL | SER | PRO | ||||
| 8 | SER | ASN | ASN | SER | LYS | GLU | ASP | ALA | PHE | SER | ||||
| 9 | GLY | THR | ASP | TRP | MET | LEU | GLU | LYS | MET | ASP | ||||
| 10 | LEU | LYS | GLU | PHE | ASP | LEU | ASP | ALA | LEU | LEU | ||||
| 11 | GLY | ILE | ASP | ASP | LEU | GLU | THR | MET | PRO | ASP | ||||
| 12 | ASP | LEU | LEU | THR | THR | LEU | ASP | ASP | THR | CYS | ||||
| 13 | ASP | LEU | PHE | ALA | PRO | LEU | VAL | GLN | GLU | THR | ||||
| 14 | ASN | LYS | GLN | PRO | PRO | GLN | THR | VAL | ASN | PRO | ||||
| 15 | ILE | GLY | HIS | LEU | PRO | GLU | SER | LEU | THR | LYS | ||||
| 16 | PRO | ASP | GLN | VAL | ALA | PRO | PHE | THR | PHE | LEU | ||||
| 17 | GLN | PRO | LEU | PRO | LEU | SER | PRO | GLY | VAL | LEU | ||||
| 18 | SER | SER | THR | PRO | ASP | HIS | SER | PHE | SER | LEU | ||||
| 19 | GLU | LEU | GLY | SER | GLU | VAL | ASP | ILE | THR | GLU | ||||
| 20 | GLY | ASP | ARG | LYS | PRO | ASP | TYR | THR | ALA | TYR | ||||
| 21 | VAL | ALA | MET | ILE | PRO | GLN | CYS | ILE | LYS | GLU | ||||
| 22 | GLU | ASP | THR | PRO | SER | ASP | ASN | ASP | SER | GLY | ||||
| 23 | ILE | CYS | MET | SER | PRO | GLU | SER | TYR | LEU | GLY | ||||
| 24 | SER | PRO | GLN | HIS | SER | PRO | SER | THR | ARG | GLY | ||||
| 25 | SER | PRO | ASN | ARG | SER | LEU | PRO | SER | PRO | GLY | ||||
| 26 | VAL | LEU | CYS | GLY | SER | ALA | ARG | PRO | LYS | PRO | ||||
| 27 | TYR | ASP | PRO | PRO | GLY | GLU | LYS | MET | VAL | ALA | ||||
| 28 | ALA | LYS | VAL | LYS | GLY |
Samples:
sample_1: ATF4 transactivation domain, [U-99% 15N], 175 uM; ATF4 transactivation domain, [U-13C; U-15N; U-2H], 175 uM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts