BMRB Entry 51075
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51075
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Citation: Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John. "Nascent chain dynamics and ribosome interactions within folded ribosome-nascent chain complexes observed by NMR spectroscopy" Chem. Sci. 12, 13120-13126 (2021).
PubMed: 34745542
Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: Dictyostelium discoideum Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLDC
Entity Sequences (FASTA):
entity_1: MHHHHHHASKPAPSAEHSYA
EGEGLVKVFDNAPAEFTIFA
VDTKGVARTDGGDPFEVAIN
GPDGLVVDAKVTDNNDGTYG
VVYDAPVEGNYNVNVTLRGN
PIKNMPIDVKCIEG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 258 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 538 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FLN5 | 1 |
Entities:
Entity 1, FLN5 114 residues - Formula weight is not available
Fifth Domain from filamin. First 9 residues are non-native affinity tag.
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | ALA | SER | LYS | ||||
| 2 | PRO | ALA | PRO | SER | ALA | GLU | HIS | SER | TYR | ALA | ||||
| 3 | GLU | GLY | GLU | GLY | LEU | VAL | LYS | VAL | PHE | ASP | ||||
| 4 | ASN | ALA | PRO | ALA | GLU | PHE | THR | ILE | PHE | ALA | ||||
| 5 | VAL | ASP | THR | LYS | GLY | VAL | ALA | ARG | THR | ASP | ||||
| 6 | GLY | GLY | ASP | PRO | PHE | GLU | VAL | ALA | ILE | ASN | ||||
| 7 | GLY | PRO | ASP | GLY | LEU | VAL | VAL | ASP | ALA | LYS | ||||
| 8 | VAL | THR | ASP | ASN | ASN | ASP | GLY | THR | TYR | GLY | ||||
| 9 | VAL | VAL | TYR | ASP | ALA | PRO | VAL | GLU | GLY | ASN | ||||
| 10 | TYR | ASN | VAL | ASN | VAL | THR | LEU | ARG | GLY | ASN | ||||
| 11 | PRO | ILE | LYS | ASN | MET | PRO | ILE | ASP | VAL | LYS | ||||
| 12 | CYS | ILE | GLU | GLY |
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