BMRB Entry 51076
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51076
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Title: Methyl side-chain chemical shift assignments for ribosomal protein bL12 PubMed: 34745542
Deposition date: 2021-09-06 Original release date: 2021-09-12
Authors: Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John
Citation: Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John. "Nascent chain dynamics and ribosome interactions within folded ribosome-nascent chain complexes observed by NMR spectroscopy" Chem. Sci. 12, 13120-13126 (2021).
Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b(+)
Entity Sequences (FASTA):
entity_1: MSITKDQIIEAVAAMSVMDV
VELISAMEEKFGVSAAAAVA
VAAGPVEAAEEKTEFDVILK
AAGANKVAVIKAVRGATGLG
LKEAKDLVESAPAALKEGVS
KDDAEALKKALEEAGAEVEV
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 14 |
| 1H chemical shifts | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | bL12 | 1 |
Entities:
Entity 1, bL12 121 residues - Formula weight is not available
E.coli ribosomal protein bL12
| 1 | MET | SER | ILE | THR | LYS | ASP | GLN | ILE | ILE | GLU | ||||
| 2 | ALA | VAL | ALA | ALA | MET | SER | VAL | MET | ASP | VAL | ||||
| 3 | VAL | GLU | LEU | ILE | SER | ALA | MET | GLU | GLU | LYS | ||||
| 4 | PHE | GLY | VAL | SER | ALA | ALA | ALA | ALA | VAL | ALA | ||||
| 5 | VAL | ALA | ALA | GLY | PRO | VAL | GLU | ALA | ALA | GLU | ||||
| 6 | GLU | LYS | THR | GLU | PHE | ASP | VAL | ILE | LEU | LYS | ||||
| 7 | ALA | ALA | GLY | ALA | ASN | LYS | VAL | ALA | VAL | ILE | ||||
| 8 | LYS | ALA | VAL | ARG | GLY | ALA | THR | GLY | LEU | GLY | ||||
| 9 | LEU | LYS | GLU | ALA | LYS | ASP | LEU | VAL | GLU | SER | ||||
| 10 | ALA | PRO | ALA | ALA | LEU | LYS | GLU | GLY | VAL | SER | ||||
| 11 | LYS | ASP | ASP | ALA | GLU | ALA | LEU | LYS | LYS | ALA | ||||
| 12 | LEU | GLU | GLU | ALA | GLY | ALA | GLU | VAL | GLU | VAL | ||||
| 13 | LYS |
Samples:
sample_1: bL12, [U-13C; U-15N], 300 uM; HEPES 10 mM; magnesium chloride 12 mM; ammonium chloride 30 mM; BME 2 mM; DSS 0.001%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4D HCccoNH TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HMQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
MATLAB - chemical shift assignment
NMRFAM-SPARKY v1.414 - chemical shift assignment
TOPSPIN - collection
NMRPipe - processing
SMILE - processing
NMR spectrometers:
- Bruker AVANCE III 700 MHz