BMRB Entry 51092
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51092
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Title: Assignment of BRCA1-A complex subunit RAP80 35-124 C70S, 97-103 poly A linker PubMed: 34764289
Deposition date: 2021-09-20 Original release date: 2021-10-01
Authors: Gaussmann, Stefan; Sattler, Michael
Citation: Fottner, Maximilian; Weyh, Maria; Gaussmann, Stefan; Schwarz, Dominic; Sattler, Michael; Lang, Kathrin. "A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes" Nat. Commun. 12, 6515-6515 (2021).
Assembly members:
entity_1, polymer, 102 residues, 11234.39 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-17b
Entity Sequences (FASTA):
entity_1: PSSHHHHHHSSGRLEDAFIV
ISDSDGEEPKEENGLQKTKT
KQSNRAKSLAKRKIAQMTEE
EQFALALKMSEQEAAAAAAA
AEEEEELLRKAIAESLNSCR
PS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 338 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RAP80 | 1 |
Entities:
Entity 1, RAP80 102 residues - 11234.39 Da.
| 1 | PRO | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | SER | ||||
| 2 | SER | GLY | ARG | LEU | GLU | ASP | ALA | PHE | ILE | VAL | ||||
| 3 | ILE | SER | ASP | SER | ASP | GLY | GLU | GLU | PRO | LYS | ||||
| 4 | GLU | GLU | ASN | GLY | LEU | GLN | LYS | THR | LYS | THR | ||||
| 5 | LYS | GLN | SER | ASN | ARG | ALA | LYS | SER | LEU | ALA | ||||
| 6 | LYS | ARG | LYS | ILE | ALA | GLN | MET | THR | GLU | GLU | ||||
| 7 | GLU | GLN | PHE | ALA | LEU | ALA | LEU | LYS | MET | SER | ||||
| 8 | GLU | GLN | GLU | ALA | ALA | ALA | ALA | ALA | ALA | ALA | ||||
| 9 | ALA | GLU | GLU | GLU | GLU | GLU | LEU | LEU | ARG | LYS | ||||
| 10 | ALA | ILE | ALA | GLU | SER | LEU | ASN | SER | CYS | ARG | ||||
| 11 | PRO | SER |
Samples:
sample_1: BRCA1-A complex subunit RAP80, [U-99% 13C; U-99% 15N], 180 uM; TRIS 20 mM; DTT 2 mM; D2O 10%; sodium chloride 100 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5 pl7 - collection
NMRPipe vLinux64:20210129 - processing
CcpNMR v2.4.2 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts