BMRB Entry 51092

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51092

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Title:
Assignment of BRCA1-A complex subunit RAP80 35-124 C70S, 97-103 poly A linker
Deposition date:
2021-09-20
Original release date:
2021-10-01
Authors:
Gaussmann, Stefan; Sattler, Michael
Citation:

Citation: Fottner, Maximilian; Weyh, Maria; Gaussmann, Stefan; Schwarz, Dominic; Sattler, Michael; Lang, Kathrin. "A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes"  Nat. Commun. 12, 6515-6515 (2021).
PubMed: 34764289

Assembly members:

Assembly members:
entity_1, polymer, 102 residues, 11234.39 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PSSHHHHHHSSGRLEDAFIV ISDSDGEEPKEENGLQKTKT KQSNRAKSLAKRKIAQMTEE EQFALALKMSEQEAAAAAAA AEEEEELLRKAIAESLNSCR PS

Data sets:
Data typeCount
13C chemical shifts338
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RAP801

Entities:

Entity 1, RAP80 102 residues - 11234.39 Da.

1   PROSERSERHISHISHISHISHISHISSER
2   SERGLYARGLEUGLUASPALAPHEILEVAL
3   ILESERASPSERASPGLYGLUGLUPROLYS
4   GLUGLUASNGLYLEUGLNLYSTHRLYSTHR
5   LYSGLNSERASNARGALALYSSERLEUALA
6   LYSARGLYSILEALAGLNMETTHRGLUGLU
7   GLUGLNPHEALALEUALALEULYSMETSER
8   GLUGLNGLUALAALAALAALAALAALAALA
9   ALAGLUGLUGLUGLUGLULEULEUARGLYS
10   ALAILEALAGLUSERLEUASNSERCYSARG
11   PROSER

Related Database Links:

SP Q96RL1
AlphaFold Q9UHX7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks