BMRB Entry 51097
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51097
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Title: Resonance assignments and Residual Dipolar Couplings for Human Beta-2 microglobulin (b2m) PubMed: 36115831
Deposition date: 2021-09-23 Original release date: 2022-10-12
Authors: Truong, Hau; Sgourakis, Nikolaos
Citation: Jiang, Jiansheng; Taylor, Daniel; Kim, Ellen; Boyd, Lisa; Ahmad, Javeed; Mage, Michael; Truong, Hau; Woodward, Claire; Sgourakis, Nikolaos; Cresswell, Peter; Margulies, David; Natarajan, Kannan. "Structural mechanism of tapasin-mediated MHC-I peptide loading in antigen presentation" Nat. Commun. 13, 5470-5470 (2022).
Assembly members:
entity_1, polymer, 100 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet22B+
Entity Sequences (FASTA):
entity_1: MIQRTPKIQVYSRHPAENGK
SNFLNCYVSGFHPSDIEVDL
LKNGERIEKVEHSDLSFSKD
WSFYLLYYTEFTPTEKDEYA
CRVNHVTLSQPKIVKWDRDM
- RDCs
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 272 |
15N chemical shifts | 87 |
1H chemical shifts | 180 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Beta-2 microglobulin | 1 |
Entities:
Entity 1, Beta-2 microglobulin 100 residues - Formula weight is not available
1 | MET | ILE | GLN | ARG | THR | PRO | LYS | ILE | GLN | VAL | |
2 | TYR | SER | ARG | HIS | PRO | ALA | GLU | ASN | GLY | LYS | |
3 | SER | ASN | PHE | LEU | ASN | CYS | TYR | VAL | SER | GLY | |
4 | PHE | HIS | PRO | SER | ASP | ILE | GLU | VAL | ASP | LEU | |
5 | LEU | LYS | ASN | GLY | GLU | ARG | ILE | GLU | LYS | VAL | |
6 | GLU | HIS | SER | ASP | LEU | SER | PHE | SER | LYS | ASP | |
7 | TRP | SER | PHE | TYR | LEU | LEU | TYR | TYR | THR | GLU | |
8 | PHE | THR | PRO | THR | GLU | LYS | ASP | GLU | TYR | ALA | |
9 | CYS | ARG | VAL | ASN | HIS | VAL | THR | LEU | SER | GLN | |
10 | PRO | LYS | ILE | VAL | LYS | TRP | ASP | ARG | ASP | MET |
Samples:
sample_1: beta-2 microglobulin, [U-13C; U-15N; U-2H], 500 uM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 1.6 mM; EDTA 5 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 150 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N TROSY | sample_1 | anisotropic | sample_conditions_2 |
Software:
NMRPipe - processing
SPARKY - chemical shift assignment
TALOS+ - data analysis
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
- Bruker AVANCE III 800 MHz
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts