BMRB Entry 51098

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51098

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Title:
Backbone and methyl resonance assignments of beta-2 microglobulin in complex with HLA-B*44:05/EEFGRAFSF
Deposition date:
2021-09-23
Original release date:
2022-10-12
Authors:
Truong, Hau; Sgourakis, Nikolaos
Citation:

Citation: Jiang, Jiansheng; Taylor, Daniel; Kim, Ellen; Boyd, Lisa; Ahmad, Javeed; Mage, Michael; Truong, Hau; Woodward, Claire; Sgourakis, Nikolaos; Cresswell, Peter; Margulies, David; Natarajan, Kannan. "Structural mechanism of tapasin-mediated MHC-I peptide loading in antigen presentation"  Nat. Commun. 13, 5470-5470 (2022).
PubMed: 36115831

Assembly members:

Assembly members:
entity_1, polymer, 277 residues, 32117.57 Da.
entity_2, polymer, 100 residues, Formula weight is not available
entity_3, polymer, 9 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet22B+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSHSMRYFYTAMSRPGRGE PRFITVGYVDDTLFVRFDSD ATSPRKEPRAPWIEQEGPEY WDRETQISKTNTQTYRENLR TALRYYNQSEAGSHIIQRMY GCDVGPDGRLLRGYDQYAYD GKDYIALNEDLSSWTAADTA AQITQRKWEAARVAEQDRAY LEGLCVESLRRYLENGKETL QRADPPKTHVTHHPISDHEV TLRCWALGFYPAEITLTWQR DGEDQTQDTELVETRPAGDR TFQKWAAVVVPSGEEQRYTC HVQHEGLPKPLTLRWEP
entity_2: MIQRTPKIQVYSRHPAENGK SNFLNCYVSGFHPSDIEVDL LKNGERIEKVEHSDLSFSKD WSFYLLYYTEFTPTEKDEYA CRVNHVTLSQPKIVKWDRDM
entity_3: EEFGRAFSF

Data sets:
Data typeCount
13C chemical shifts294
15N chemical shifts88
1H chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HLA-B*44:051
2beta-2 microglobulin2
3peptide3

Entities:

Entity 1, HLA-B*44:05 277 residues - 32117.57 Da.

1   METGLYSERHISSERMETARGTYRPHETYR
2   THRALAMETSERARGPROGLYARGGLYGLU
3   PROARGPHEILETHRVALGLYTYRVALASP
4   ASPTHRLEUPHEVALARGPHEASPSERASP
5   ALATHRSERPROARGLYSGLUPROARGALA
6   PROTRPILEGLUGLNGLUGLYPROGLUTYR
7   TRPASPARGGLUTHRGLNILESERLYSTHR
8   ASNTHRGLNTHRTYRARGGLUASNLEUARG
9   THRALALEUARGTYRTYRASNGLNSERGLU
10   ALAGLYSERHISILEILEGLNARGMETTYR
11   GLYCYSASPVALGLYPROASPGLYARGLEU
12   LEUARGGLYTYRASPGLNTYRALATYRASP
13   GLYLYSASPTYRILEALALEUASNGLUASP
14   LEUSERSERTRPTHRALAALAASPTHRALA
15   ALAGLNILETHRGLNARGLYSTRPGLUALA
16   ALAARGVALALAGLUGLNASPARGALATYR
17   LEUGLUGLYLEUCYSVALGLUSERLEUARG
18   ARGTYRLEUGLUASNGLYLYSGLUTHRLEU
19   GLNARGALAASPPROPROLYSTHRHISVAL
20   THRHISHISPROILESERASPHISGLUVAL
21   THRLEUARGCYSTRPALALEUGLYPHETYR
22   PROALAGLUILETHRLEUTHRTRPGLNARG
23   ASPGLYGLUASPGLNTHRGLNASPTHRGLU
24   LEUVALGLUTHRARGPROALAGLYASPARG
25   THRPHEGLNLYSTRPALAALAVALVALVAL
26   PROSERGLYGLUGLUGLNARGTYRTHRCYS
27   HISVALGLNHISGLUGLYLEUPROLYSPRO
28   LEUTHRLEUARGTRPGLUPRO

Entity 2, beta-2 microglobulin 100 residues - Formula weight is not available

1   METILEGLNARGTHRPROLYSILEGLNVAL
2   TYRSERARGHISPROALAGLUASNGLYLYS
3   SERASNPHELEUASNCYSTYRVALSERGLY
4   PHEHISPROSERASPILEGLUVALASPLEU
5   LEULYSASNGLYGLUARGILEGLULYSVAL
6   GLUHISSERASPLEUSERPHESERLYSASP
7   TRPSERPHETYRLEULEUTYRTYRTHRGLU
8   PHETHRPROTHRGLULYSASPGLUTYRALA
9   CYSARGVALASNHISVALTHRLEUSERGLN
10   PROLYSILEVALLYSTRPASPARGASPMET

Entity 3, peptide 9 residues - Formula weight is not available

1   GLUGLUPHEGLYARGALAPHESERPHE

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks